Document Detail


Antisense RNA-dependent transcription termination sites that modulate lysogenic development of satellite phage P4.
MedLine Citation:
PMID:  10844696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the lysogenic state, bacteriophage P4 prevents the expression of its own replication genes, which are encoded in the left operon, through premature transcription termination. The phage factor responsible for efficient termination is a small, untranslated RNA (CI RNA), which acts as an antisense RNA and controls transcription termination by pairing with two complementary sequences (seqA and seqC) located within the leader region of the left operon. A Rho-dependent termination site, timm, was previously shown to be involved in the control of P4 replication gene expression. In the present study, by making use of phage PhiR73 as a cloning vector and of suppressor tRNAGly as a reporter gene, we characterized two additional terminators, t1 and t4. Although transcription termination at neither site requires the Rho factor, only t1 has the typical structure of a Rho-independent terminator. t1 is located between the PLE promoter and the cI gene, whereas t4 is located between cI and timm. Efficient termination at t1 requires the CI RNA and the seqA target sequence; in vitro, the CI RNA enhanced termination at t1 in the absence of any bacterial factor. A P4 mutant, in which the t1 terminator has been deleted, can still lysogenize both Rho+ and Rho- strains and exhibits increased expression of CI RNA. These data indicate that t1 and the Rho-dependent timm terminators are not essential for lysogeny. t1 is involved in CI RNA autoregulation, whereas t4 appears to be the main terminator necessary to prevent expression of the lytic genes in the lysogenic state.
Authors:
F Briani; D Ghisotti; G Dehò
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  36     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-08-15     Completed Date:  2000-08-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1124-34     Citation Subset:  IM    
Affiliation:
Dipartimento di Genetica e di Biologia dei Microrganismi, Università degli Studi di Milano, Milan, Italy.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Outer Membrane Proteins
Bacterial Proteins / metabolism
Coliphages / genetics*
DNA-Binding Proteins*
Escherichia coli Proteins
Nucleic Acid Conformation
RNA, Antisense*
Repressor Proteins / genetics*
Terminator Regions, Genetic*
Transcription Factors*
Transcription, Genetic*
Viral Proteins
Viral Regulatory and Accessory Proteins
rho GTP-Binding Proteins / metabolism
Chemical
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/Escherichia coli Proteins; 0/RNA, Antisense; 0/Repressor Proteins; 0/SeqA protein, E coli; 0/Transcription Factors; 0/Viral Proteins; 0/Viral Regulatory and Accessory Proteins; 0/phage repressor proteins; EC 3.6.5.2/rho GTP-Binding Proteins

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