Document Detail

Antiretroviral effect of 4-thio-uridylate against human immunodeficiency virus type 1.
MedLine Citation:
PMID:  23195558     Owner:  NLM     Status:  In-Data-Review    
Antiretroviral effect of thiolated nucleotide 4-thio-uridylate (S4UMP, designated as UD29) against human immunodeficiency virus type 1 (HIV-1) have been quantitatively determined in cell-based viral infectivity assays. In syntitium inhibition assay on MT-2 human T-cell line UD29 prevented cell fusion and formation of syntitia induced by HIV-1IIIB with IC50 values of 11.7 μg/ml. In a single-cycle viral infection assay (MAGI assay) UD29 proved to have a potent inhibitory effect against HIV-1IIIB on HeLaCD4-LTR/β-gal cells, which was dose dependent with IC50 values of 4.75 μg/ml and IC90 of 39.7 μg/ml. UD29 showed a most prominent antiviral effect when administered 30 min prior HIV-1 infection. As HIV entry requires thiol/disulfide exchange process, results suggest that reactive -SH group of enol-form of the thiolated nucleotide may interfere with the function of cell surface proteins. UD29 cannot penetrate into cells and may have an interactive role in redox processes active in viral entry.
Szilvia Kanizsai; Agoston Ghidán; Joseph Ongrádi; Károly Nagy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta microbiologica et immunologica Hungarica     Volume:  59     ISSN:  1217-8950     ISO Abbreviation:  Acta Microbiol Immunol Hung     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9434021     Medline TA:  Acta Microbiol Immunol Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  499-510     Citation Subset:  IM    
Semmelweis University Institute of Medical Microbiology Budapest Hungary.
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