| Antiproliferative and proapoptotic effects of somatostatin on activated hepatic stellate cells. | |
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MedLine Citation:
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PMID: 15052685 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To assess the effects of somatostatin on proliferation and apoptosis of activated rat hepatic stellate cells (HSCs). METHODS: HSCs isolated from the livers of adult Sprague-Dawley rats (weighing 400-500 g) by in situ perfusion and purified by single-step density gradient centrifugation with Nycodenz, became activated after 10 days' cultivation. Then the apoptotic rate of HSCs treated with different doses of somatostatin for 72 h, was assayed by acridine orange/ethidium bromide fluorescent staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, transmission electron microscopy and flow cytometry, while the proliferation of HSCs was measured by MTT assay. Furthermore, the mechanisms of somatostatin were investigated by cytodynamic analysis. RESULTS: Somatostatin at the concentration of 10(-6)-10(-9) mol/L could decrease the proliferative rate, and promote the apoptosis of activated rat HSCs in a dose-dependent way. Its action was most significant when the concentration reached 10(-6) mol/L or 10(-7) mol/L (P<0.05-0.01). An obvious cell-cycle arrest (G(0)/G(1) arrest) was the important way for somatostatin to exert its action. CONCLUSION: Antiproliferative and proapoptotic effects of low-dose somatostatin on activated rat HSCs can be obtained. These findings reveal its potential antifibrotic action. |
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Authors:
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Qin Pan; Ding-Guo Li; Han-Ming Lu; Liang-Yong Lu; Yu-Qin Wang; Qin-Fang Xu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 10 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2004 Apr |
Date Detail:
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Created Date: 2004-03-30 Completed Date: 2004-05-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 1015-8 Citation Subset: IM |
Affiliation:
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Department of Gastroenterology, Shanghai Second Medical University, Xinhua Hospital, 1665 KongJiang Rd., Shanghai 200092, China. fangchunhua@online.sh.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Cell Division / drug effects Cells, Cultured Hepatocytes / cytology*, drug effects, physiology* Male Rats Rats, Sprague-Dawley Somatostatin / pharmacology* |
| Chemical | |
Reg. No./Substance:
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51110-01-1/Somatostatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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