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Antiproliferative effects of selected chemotherapeutics in human ovarian cancer cell line A2780.
MedLine Citation:
PMID:  23297519     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
The aim of our study was to determine the effect of selected cytostatics on a human ovarian cancer cell line A2780 as a model system for ovarian cancer treatment. This cell line is considered cisplatin-sensitive. Panel of tested cytostatics included cisplatin, paclitaxel, carboplatin, gemcitabine, topotecan and etoposide. These cytostatics have a different mechanism of action. To evaluate cytotoxic potential of the tested compounds, the methods measuring various toxicological endpoints were employed including morphological studies, MTT assay, dynamic monitoring of cell proliferation with xCELLigence, cell cycle analysis, caspase 3 activity and expression of proteins involved in cell cycle regulation and cell death. The A270 cell line showed different sensitivity towards the selected cytostatics, the highest cytotoxic effect was associated with paclitaxel and topotecan.
Authors:
Katerina Caltová; Miroslav Cervinka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta medica (Hradec Králové) / Universitas Carolina, Facultas Medica Hradec Králové     Volume:  55     ISSN:  1211-4286     ISO Abbreviation:  Acta Medica (Hradec Kralove)     Publication Date:  2012  
Date Detail:
Created Date:  2013-01-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9705947     Medline TA:  Acta Medica (Hradec Kralove)     Country:  Czech Republic    
Other Details:
Languages:  eng     Pagination:  116-24     Citation Subset:  IM    
Affiliation:
Department of Medical Biology and Genetics, Charles University in Prague, Faculty of Medicine, Hradec Králové, Czech Republic. brigulovak@lfhk.cuni.cz
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