Document Detail


Antiproliferative effects of preservative-free triamcinolone acetonide on cultured human retinal pigment epithelial cells.
MedLine Citation:
PMID:  19644411     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The control of retinal pigment epithelial cell proliferation is an essential factor in the clinical management of proliferative vitreoretinopathy (PVR). Factors inhibiting PVR without toxic potential are of special interest in ophthalmology. The aim of the study was to investigate the antiproliferative and cytotoxic effects of a preservative-free triamcinolone acetonide (PFTA) suspension on a human retinal pigment epithelial (ARPE19) cell line in vitro. MATERIAL/METHODS: ARPE19 cells (immortal non-transformed cells from a human donor) were seeded and incubated in vitro with increasing concentrations of PFTA (0.01-1 mg/ml). After 1, 3, and 7 days, cellular proliferative activity was assessed by 5'-bromo-2'-deoxyuridine (BrdU) incorporation into cellular DNA and cell proliferation was determined using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. To determine cytotoxicity, ARPE19 cells were grown to confluence and subsequently cultured in serum-deficient medium to ensure a static milieu and the MTT test was performed after 24 hours of incubation with PFTA. Cell recovery after transient PFTA exposure was also compared with continuous exposure after 7 days. RESULTS: PFTA inhibits ARPE19 cell proliferation in a dose-dependent manner. Significant inhibition of cell proliferation was observed on the first day of the study at 1, 0.1, and 0.01 mg/ml PFTA and significant reductions in ARPE19 cells were noted for 1 and 0.1 mg/ml PFTA. Proliferation was resumed in all ARPE19 cultures and was dependent on the initial PFTA concentration. PFTA did not cause a cytotoxic effect. CONCLUSIONS: PFTA influences the proliferation of vital ARPE19 cells in a reversible manner without cytotoxic effect.
Authors:
Radosław Kaczmarek; Peter Szurman; Marta Misiuk-Hojło; Andrzej Grzybowski
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical science monitor : international medical journal of experimental and clinical research     Volume:  15     ISSN:  1643-3750     ISO Abbreviation:  Med. Sci. Monit.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-31     Completed Date:  2009-10-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9609063     Medline TA:  Med Sci Monit     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  BR227-31     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, Wroclaw Medical University, Wrocław, Poland.
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MeSH Terms
Descriptor/Qualifier:
Biological Assay
Cell Death / drug effects
Cell Proliferation / drug effects
Cell Survival / drug effects
Cells, Cultured
Epithelial Cells / cytology*,  drug effects*
Humans
Pigment Epithelium of Eye / cytology*
Preservatives, Pharmaceutical
Triamcinolone Acetonide / pharmacology*
Chemical
Reg. No./Substance:
0/Preservatives, Pharmaceutical; 76-25-5/Triamcinolone Acetonide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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