| Antiproliferative effects of preservative-free triamcinolone acetonide on cultured human retinal pigment epithelial cells. | |
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MedLine Citation:
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PMID: 19644411 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The control of retinal pigment epithelial cell proliferation is an essential factor in the clinical management of proliferative vitreoretinopathy (PVR). Factors inhibiting PVR without toxic potential are of special interest in ophthalmology. The aim of the study was to investigate the antiproliferative and cytotoxic effects of a preservative-free triamcinolone acetonide (PFTA) suspension on a human retinal pigment epithelial (ARPE19) cell line in vitro. MATERIAL/METHODS: ARPE19 cells (immortal non-transformed cells from a human donor) were seeded and incubated in vitro with increasing concentrations of PFTA (0.01-1 mg/ml). After 1, 3, and 7 days, cellular proliferative activity was assessed by 5'-bromo-2'-deoxyuridine (BrdU) incorporation into cellular DNA and cell proliferation was determined using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. To determine cytotoxicity, ARPE19 cells were grown to confluence and subsequently cultured in serum-deficient medium to ensure a static milieu and the MTT test was performed after 24 hours of incubation with PFTA. Cell recovery after transient PFTA exposure was also compared with continuous exposure after 7 days. RESULTS: PFTA inhibits ARPE19 cell proliferation in a dose-dependent manner. Significant inhibition of cell proliferation was observed on the first day of the study at 1, 0.1, and 0.01 mg/ml PFTA and significant reductions in ARPE19 cells were noted for 1 and 0.1 mg/ml PFTA. Proliferation was resumed in all ARPE19 cultures and was dependent on the initial PFTA concentration. PFTA did not cause a cytotoxic effect. CONCLUSIONS: PFTA influences the proliferation of vital ARPE19 cells in a reversible manner without cytotoxic effect. |
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Authors:
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Radosław Kaczmarek; Peter Szurman; Marta Misiuk-Hojło; Andrzej Grzybowski |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Medical science monitor : international medical journal of experimental and clinical research Volume: 15 ISSN: 1643-3750 ISO Abbreviation: Med. Sci. Monit. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-31 Completed Date: 2009-10-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9609063 Medline TA: Med Sci Monit Country: Poland |
Other Details:
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Languages: eng Pagination: BR227-31 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Wroclaw Medical University, Wrocław, Poland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Assay Cell Death / drug effects Cell Proliferation / drug effects Cell Survival / drug effects Cells, Cultured Epithelial Cells / cytology*, drug effects* Humans Pigment Epithelium of Eye / cytology* Preservatives, Pharmaceutical Triamcinolone Acetonide / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Preservatives, Pharmaceutical; 76-25-5/Triamcinolone Acetonide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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