Document Detail


Antiproliferative effects of the biologically active metabolite of vitamin D3 (1,25 [OH]2 D3) on head and neck squamous cell carcinoma cell lines.
MedLine Citation:
PMID:  8858258     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In addition to a role in calcium and phosphate homeostasis other vitamin D receptor (VDR) mediated effects have been discovered during the past few years for the biologically active metabolite of vitamin D, 1,25(OH)2D3. An antiproliferative, differentiation-inducing effect on non-malignant and neoplastic cells of different origin has now been described. We examined the influence of 1,25(OH)2D3 on human squamous cell carcinomas of the head and neck (SCCHN). A differentiated (JP-PA) and undifferentiated (LF-FR) SCCHN line was studied with respect to proliferative capacity (using [3H]-thymidine uptake and cell number) and cell cycle distribution as determined by flow cytometry (FACS). Both cell lines were positive for VDR, which was found to be increased after the addition of 10(-7) M 1,25(OH)2D3, as shown by FACS analyses. The administration of 1,25(OH)2D3 at a concentration between 10(-7) M and 10(-10) M caused a dose-dependent moderate growth inhibition, as reflected by down-regulation of DNA synthesis (reduced [3H]-thymidine uptake) and a decrease in cell numbers. The JP-PA cell line showed a significant growth reduction for both concentrations tested, whereas for LF-FR a significant inhibition was detected only for 10(-7) M. The addition of 10(-7) M 1,25(OH)2D3 caused a blockade in the transition of cells from G1 to S phase in both cell lines, with a significant accumulation of cells in the G0/G1 phase. Our results demonstrate a receptor-mediated, dose-dependent inhibition of neoplastic growth by 1,25(OH)2D3 in human SCCHN lines.
Authors:
J Kornfehl; M Formanek; A Temmel; B Knerer; M Willheim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery     Volume:  253     ISSN:  0937-4477     ISO Abbreviation:  Eur Arch Otorhinolaryngol     Publication Date:  1996  
Date Detail:
Created Date:  1996-12-24     Completed Date:  1996-12-24     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9002937     Medline TA:  Eur Arch Otorhinolaryngol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  341-4     Citation Subset:  IM    
Affiliation:
Department of Oto-Rhino-Laryngology, University of Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Squamous Cell / chemistry,  drug therapy,  pathology*
Cell Cycle / drug effects
Cell Division / drug effects
Cholecalciferol / administration & dosage,  pharmacology*
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Flow Cytometry
Head and Neck Neoplasms / chemistry,  drug therapy,  pathology*
Humans
Receptors, Calcitriol* / analysis,  drug effects
Tumor Cells, Cultured / drug effects
Chemical
Reg. No./Substance:
0/Receptors, Calcitriol; 67-97-0/Cholecalciferol

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