Document Detail

Antiproliferative effects and apoptosis induction by probiotic cytoplasmic extracts in fish cell lines.
MedLine Citation:
PMID:  17706899     Owner:  NLM     Status:  MEDLINE    
Probiotic bacteria are known to exert a wide range of beneficial effects on their animal hosts. Control of intestinal homeostasis, inflammation suppression and a reduction in the incidence of cancer all rely on the antiproliferative potential of probiotics. In this paper, we assess the antiproliferative activity of probiotics in two teleost fish cell lines SAF-1, a fibroblast cell line and EPC, an epithelioma from carp. Cells were grown in the presence of cytoplasmic extracts obtained from two bacterial strains, Lactobacillus delbrüeckii subsp. lactis (LDL) and 51M6. Proliferation and apoptosis were measured after 4, 24, 48 or 72h in culture by the crystal violet or by double staining flow cytometry assays, respectively. Generally, LDL had stronger effects on cell growth than 51M6. Moreover, SAF-1 cells were more susceptible to growth inhibition than EPC cells. Apoptosis took place following growth inhibition, especially when LDL extracts were used. The results are discussed in terms of the biological significance of probiotic bacteria that naturally occur on the fish mucosal surfaces with an emphasis on how dose and species specificity may be determinant factors.
Irene Salinas; José Meseguer; Maria Angeles Esteban
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-10
Journal Detail:
Title:  Veterinary microbiology     Volume:  126     ISSN:  0378-1135     ISO Abbreviation:  Vet. Microbiol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-11-23     Completed Date:  2008-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  287-94     Citation Subset:  IM    
Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain.
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MeSH Terms
Apoptosis / drug effects*
Cell Line
Cell Proliferation / drug effects
Cytoplasm / chemistry*
Epithelial Cells / cytology,  drug effects
Fibroblasts / cytology,  drug effects
Lactobacillus delbrueckii / cytology*
Probiotics / pharmacology*
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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