Document Detail

Antiproliferative effect on human cancer cell lines after treatment with nimbolide extracted from an edible part of the neem tree (Azadirachta indica).
MedLine Citation:
PMID:  17163581     Owner:  NLM     Status:  MEDLINE    
Nimbolide, a triterpenoid extracted from the flowers of the neem tree (Azadirachta indica), was found to have antiproliferative activity against some cancer cell lines. Treatment of cells with 0.5-5.0 microm concentrations of nimbolide resulted in moderate to very strong growth inhibition in U937, HL-60, THP1 and B16 cell lines. Flow cytometric analysis of U937 cells showed that nimbolide treatment (1-2.5 microm) resulted in cell cycle disruption by decreasing the number of cells in G0/G1 phase, with initial increases in S and G2/M phases. Cells exposed to a higher dose of nimbolide for a longer period displayed a severely damaged DNA profile, resulting in a remarkable increase in the number of cells in the sub-G1 fraction, with a reciprocal decrease of cells in all phases. Quantification of the expression of phosphatidylserine in the outer cell membrane showed that doses of nimbolide higher than 0.4 microm exerted remarkable lethality, with over 60% of cells exhibiting apoptotic features after exposure to 1.2 microm nimbolide. The antiproliferative effect of nimbolide and its apoptosis-inducing property raise hope for its use in anticancer therapy by enhancing the effectiveness of cell cycle disruption.
Molay Kumar Roy; Masuko Kobori; Makiko Takenaka; Kazuhiko Nakahara; Hiroshi Shinmoto; Seiichiro Isobe; Tojiro Tsushida
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Phytotherapy research : PTR     Volume:  21     ISSN:  0951-418X     ISO Abbreviation:  Phytother Res     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-08     Completed Date:  2007-05-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8904486     Medline TA:  Phytother Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  245-50     Citation Subset:  IM    
National Food Research Institute, 2-1-12, Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
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MeSH Terms
Antineoplastic Agents, Phytogenic / administration & dosage,  pharmacology*,  therapeutic use
Cell Cycle / drug effects
Cell Line, Tumor / drug effects
Cell Proliferation / drug effects
Flow Cytometry
Limonins / administration & dosage,  pharmacology*,  therapeutic use
Plant Extracts / administration & dosage,  pharmacology,  therapeutic use
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Limonins; 0/Plant Extracts; 25990-37-8/nimbolide

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