| Antiproliferative effect of flavomannin-6,6'-dimethylether from Tricholoma equestre on Caco-2 cells. | |
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MedLine Citation:
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PMID: 19686795 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several studies have been performed reporting antitumoral activity of different mushroom extracts. The current study reports the antiproliferative activity of flavomannin-6,6'-dimethylether obtained from a very common edible mushroom: Tricholoma equestre(L.)P.Kumm, and the characterization of its effects at molecular level. Concentrations causing 50% and 80% growth inhibition on human adenocarcinoma colorectal Caco-2 cells were determined (in microg/mL: IC(50)=96+/-3 after 24 h and 78+/-7 after 48 h, IC(80)=112+/-4 after 24 h and 90+/-3 after 48 h) by using MTT method. It was demonstrated that flavomannin-6,6'-dimethylether induced an arrest in G0/G1 phase of the cell cycle by flow cytometry analysis and an increase of p27 protein level by Western blot. Furthermore, this compound did not induce apoptosis by flow cytometry or DNA fragmentation by gel electrophoresis. Thus, it could be a promising agent due to its cytostatic effect against Caco-2 tumoral cells, and the absence of a genotoxic effect. |
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Authors:
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Gisela Pachón-Peña; Fernando J Reyes-Zurita; Gerard Deffieux; Amaia Azqueta; Adela López de Cerain; Josep J Centelles; Edmond E Creppy; Marta Cascante |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-15 |
Journal Detail:
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Title: Toxicology Volume: 264 ISSN: 1879-3185 ISO Abbreviation: Toxicology Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-09-28 Completed Date: 2009-10-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0361055 Medline TA: Toxicology Country: Ireland |
Other Details:
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Languages: eng Pagination: 192-7 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB) and Unit Associated with CSIC, Diagonal 645, Barcelona, Spain. giselapachon@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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metabolism,
pathology* Anthracenes / isolation & purification, pharmacology* Antineoplastic Agents / chemistry*, isolation & purification Apoptosis / drug effects Caco-2 Cells Cell Cycle / drug effects Cell Proliferation / drug effects* Colorectal Neoplasms / metabolism, pathology* DNA Fragmentation Dose-Response Relationship, Drug Humans Inhibitory Concentration 50 Intracellular Signaling Peptides and Proteins / metabolism Time Factors Tricholoma / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Anthracenes; 0/Antineoplastic Agents; 0/CDKN1B protein, human; 0/Intracellular Signaling Peptides and Proteins; 0/flavomannin-6,6'-dimethylether |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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