Document Detail


Antiproliferative effect of flavomannin-6,6'-dimethylether from Tricholoma equestre on Caco-2 cells.
MedLine Citation:
PMID:  19686795     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several studies have been performed reporting antitumoral activity of different mushroom extracts. The current study reports the antiproliferative activity of flavomannin-6,6'-dimethylether obtained from a very common edible mushroom: Tricholoma equestre(L.)P.Kumm, and the characterization of its effects at molecular level. Concentrations causing 50% and 80% growth inhibition on human adenocarcinoma colorectal Caco-2 cells were determined (in microg/mL: IC(50)=96+/-3 after 24 h and 78+/-7 after 48 h, IC(80)=112+/-4 after 24 h and 90+/-3 after 48 h) by using MTT method. It was demonstrated that flavomannin-6,6'-dimethylether induced an arrest in G0/G1 phase of the cell cycle by flow cytometry analysis and an increase of p27 protein level by Western blot. Furthermore, this compound did not induce apoptosis by flow cytometry or DNA fragmentation by gel electrophoresis. Thus, it could be a promising agent due to its cytostatic effect against Caco-2 tumoral cells, and the absence of a genotoxic effect.
Authors:
Gisela Pachón-Peña; Fernando J Reyes-Zurita; Gerard Deffieux; Amaia Azqueta; Adela López de Cerain; Josep J Centelles; Edmond E Creppy; Marta Cascante
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-15
Journal Detail:
Title:  Toxicology     Volume:  264     ISSN:  1879-3185     ISO Abbreviation:  Toxicology     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-28     Completed Date:  2009-10-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0361055     Medline TA:  Toxicology     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  192-7     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB) and Unit Associated with CSIC, Diagonal 645, Barcelona, Spain. giselapachon@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / metabolism,  pathology*
Anthracenes / isolation & purification,  pharmacology*
Antineoplastic Agents / chemistry*,  isolation & purification
Apoptosis / drug effects
Caco-2 Cells
Cell Cycle / drug effects
Cell Proliferation / drug effects*
Colorectal Neoplasms / metabolism,  pathology*
DNA Fragmentation
Dose-Response Relationship, Drug
Humans
Inhibitory Concentration 50
Intracellular Signaling Peptides and Proteins / metabolism
Time Factors
Tricholoma / chemistry*
Chemical
Reg. No./Substance:
0/Anthracenes; 0/Antineoplastic Agents; 0/CDKN1B protein, human; 0/Intracellular Signaling Peptides and Proteins; 0/flavomannin-6,6'-dimethylether

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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