Document Detail


Antiproliferative and apoptotic effects of tetrandrine on different human hepatoma cell lines.
MedLine Citation:
PMID:  16437745     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tetrandrine (TET), a bis-benzylisoquinoline alkaloid isolated from the dried root of Hang-Fang-Chi (Stephania tetrandra S. Moore), is well known to possess activities including antioxidant, anti-inflammation, anti-fibrotic and anticancer. It is used clinically to treat hypertension and silicosis. In the present study, the anti-proliferative and apoptotic effects of TET were evaluated on three different hepatoma cell lines, namely Hep G2, PLC/PRF/5 and Hep 3B. Using XTT assay, results showed that the IC50 values of TET were 4.35 microM for Hep G2, 9.44 microM for PLC/PRF/5 and 10.41 microM for Hep 3B cells. The CC50 of TET against BNL-CL.2 mouse normal liver cells was 31.12 microM. Interestingly, TET exhibited a lower IC50 value and better selectivity against Hep G2 and PLC/PRF/5 cells than cisplatin. Microscopic observation study, DNA fragmentation assay and flow cytometric analysis further supported apoptotic effect of TET on both PLC/PRF/5 and Hep 3B cells. The cell cycle of PLC/PRF/5 treated with TET appeared to arrest at G2/M phase in a dose-dependent manner, whereas no effect was noted on the cell cycle of Hep 3B cells. The present study concludes that TET exhibited anti-proliferative effect on Hep G2, PLC/PRF/5 and Hep 3B cells in a dose-dependent manner. TET also possesses a lower IC50 and better SI value than cisplatin against Hep G2 and PLC/PRF/5 cells. The effect of TET on cell cycle progression was found to vary with the type of hepatoma cells, suggesting the genetic make-up of the cells play an important role in the response to drug treatment.
Authors:
Lean Teik Ng; Lien-Chai Chiang; Yu-Tseng Lin; Chun-Ching Lin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of Chinese medicine     Volume:  34     ISSN:  0192-415X     ISO Abbreviation:  Am. J. Chin. Med.     Publication Date:  2006  
Date Detail:
Created Date:  2006-01-26     Completed Date:  2006-08-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7901431     Medline TA:  Am J Chin Med     Country:  Singapore    
Other Details:
Languages:  eng     Pagination:  125-35     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, Tajen University, Pingtung, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Alkaloids / chemistry,  pharmacology*
Animals
Antineoplastic Agents, Phytogenic / chemistry,  pharmacology*
Apoptosis / drug effects*
Benzylisoquinolines / chemistry,  pharmacology*
Carcinoma, Hepatocellular / drug therapy,  pathology*
Cell Line
Cell Proliferation / drug effects*
DNA Fragmentation / drug effects
Dose-Response Relationship, Drug
Drugs, Chinese Herbal / chemistry,  pharmacology
Humans
Interphase / drug effects
Liver Neoplasms / drug therapy,  pathology*
Mice
Molecular Structure
Chemical
Reg. No./Substance:
0/Alkaloids; 0/Antineoplastic Agents, Phytogenic; 0/Benzylisoquinolines; 0/Drugs, Chinese Herbal; 518-34-3/tetrandrine

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