| Antiproliferative activity and standardization of Tecomella undulata bark extract on K562 cells. | |
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MedLine Citation:
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PMID: 21843623 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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ETHNOPHARMACOLOGICAL RELEVANCE: The bark of Tecomella undulata is primarily used in the treatment of syphilis, painful swellings and cancer by traditional healers. Also, it is claimed to be useful in treating urinary discharges, enlargement of spleen, leucorrhoea, leukoderma, tumors, liver disorders, gonorrhea, gout and promotes wound healing in Indian traditional system of medicine. AIM: To establish a scientific validation for the antitumor effects of Tecomella undulata bark and explore the mechanistic pathway in chronic myeloid leukemia cell line, K562. The study was further extended to standardize the extract using quercetin as biomarker. METHODS: Induction of apoptosis by chloroform extract of Tecomella undulata bark (CTUB) was determined by MTT, Annexin V and caspase activation assays. The cell cycle analysis was done by flow cytometer and nuclear staining by DAPI. The standardization of the extract was performed through reverse phase-HPLC method under PDA detection. RESULT: Results clearly showed the induction of apoptosis by CTUB in K562 cells. The effect was found to be dose dependent, having IC(50) of 30μg/ml with activation of FAS, FADD, caspase 8, caspase 3/7 and fragmentation of DNA. The bioactive CTUB was determined to possess 0.03% (w/w) of quercetin. CONCLUSION: The investigation clearly demonstrated the potential antitumor effect of CTUB, thereby validating the traditional claim. Quercetin, known to have anticancer activity is being reported and quantified for the first time from the bark of Tecomella undulata. |
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Authors:
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Alvala Ravi; A Mallika; Venkatesh Sama; A Sajeli Begum; Rukaiyya S Khan; B Madhava Reddy |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-8-6 |
Journal Detail:
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Title: Journal of ethnopharmacology Volume: - ISSN: 1872-7573 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-8-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7903310 Medline TA: J Ethnopharmacol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Ireland Ltd. |
Affiliation:
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G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad-500 028, AP, India. |
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