| Antiproliferative activity of CCN3: involvement of the C-terminal module and post-translational regulation. | |
| | |
MedLine Citation:
|
PMID: 17340618 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Previous work had suggested that recombinant CCN3 was partially inhibiting cell proliferation. Here we show that native CCN3 protein secreted into the conditioned medium of glioma transfected cells indeed induces a reduction in cell proliferation. Large amounts of CCN3 are shown to accumulate both cytoplasmically and extracellularly as cells reach high density, therefore highlighting new aspects on how cell growth may be regulated by CCN proteins. Evidence is presented establishing that the amount of CCN3 secreted into cell culture medium is regulated by post-translational proteolysis. As a consequence, the production of CCN3 varies throughout the cell cycle and CCN3 accumulates at the G2/M transition of the cycle. We also show that CCN3-induced inhibition of cell growth can be partially reversed by specific antibodies raised against a C-terminal peptide of CCN3. The use of several clones expressing various portions of CCN3 established that the CT module of CCN3 is sufficient to induce cell growth inhibition. |
| | |
Authors:
|
A M Bleau; N Planque; N Lazar; D Zambelli; A Ori; T Quan; G Fisher; K Scotlandi; B Perbal |
Related Documents
:
|
15215208 - Zyg-11 and cul-2 regulate progression through meiosis ii and polarity establishment in ... 19066628 - Enhanced proliferation of monolayer cultures of embryonic stem (es) cell-derived cardio... 1891178 - Flowcytometric measurement of the cell cycle of experimental tumors: some devices for a... 19426078 - Can the solar cycle and climate synchronize the snowshoe hare cycle in canada? evidence... 9627698 - Reduction of epidermal abnormalities and inflammatory changes in psoriatic plaques duri... 15608628 - Microfluidic sorting of mammalian cells by optical force switching. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of cellular biochemistry Volume: 101 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2007 Aug |
Date Detail:
|
Created Date: 2007-07-31 Completed Date: 2008-04-09 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
|
Languages: eng Pagination: 1475-91 Citation Subset: IM |
Affiliation:
|
Université Paris7-D. Diderot, UFR de Biochimie, Laboratoire d'Oncologie Virale et Moléculaire, 2 place Jussieu, 75005 Paris, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Cycle / physiology Cell Line Cell Proliferation* Connective Tissue Growth Factor Culture Media / chemistry Gene Expression Regulation Humans Immediate-Early Proteins / chemistry, genetics, metabolism* Intercellular Signaling Peptides and Proteins / chemistry, genetics, metabolism* Nephroblastoma Overexpressed Protein Protein Processing, Post-Translational* Protein Structure, Tertiary Recombinant Proteins / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/CTGF protein, human; 0/Culture Media; 0/Immediate-Early Proteins; 0/Intercellular Signaling Peptides and Proteins; 0/NOV protein, human; 0/Nephroblastoma Overexpressed Protein; 0/Recombinant Proteins; 139568-91-5/Connective Tissue Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evidence for the existence of satellite DNA-containing connection between metaphase chromosomes.
Next Document: Identification of a monopartite sequence in PU.1 essential for nuclear import, DNA-binding and trans...