Document Detail


Antiproliferative activity of 3-aminobenzamide in A431 carcinoma cells is associated with a target effect on cytoskeleton.
MedLine Citation:
PMID:  8780697     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
3-Aminobenzamide (3-ABA) is an inhibitor of poly(ADP-ribose)polymerase (PARP), an enzyme involved in several cellular processes, and exerts its effects by acting at the cytoskeleton level. Here we show that 3-ABA has an antiproliferative effect on the human carcinoma cell line A431, as measured by different assays. 3-ABA was capable of inhibiting cell growth as well as colony formation, this inhibitory effect is reversible. Morphological analyses showed a series of cellular alterations, such as a remarkable increase of dendritic-like protrusions, quite unusual in epithelial cells, and suggestive of a differentiative triggering. Immunocytochemical studies suggested that a major target of 3-ABA was indeed the cytoskeleton. These data, together with those of the literature, indicate that 3-ABA, depending on cell histotype and drug concentration, is a versatile drug capable of exerting antiproliferative and cytostatic effects as well as cytotoxic and antiapoptotic effects, processes sharing an important involvement of cytoskeleton. These unique characteristics of 3-ABA may be of interest for cancer research.
Authors:
R Tiozzo; D Monti; E Straface; M Capri; M A Croce; G Rainaldi; C Franceschi; W Malorni
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  225     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-10-22     Completed Date:  1996-10-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  826-32     Citation Subset:  IM    
Affiliation:
Department of Biomedical Science, University of Modena, Italy.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Benzamides / pharmacology*
Cell Division / drug effects*
Cell Survival / drug effects
Cytoskeleton / drug effects*,  metabolism
Enzyme Inhibitors / pharmacology*
Humans
Poly(ADP-ribose) Polymerases / antagonists & inhibitors*
Tubulin / metabolism
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Actins; 0/Benzamides; 0/Enzyme Inhibitors; 0/Tubulin; 3544-24-9/3-aminobenzamide; EC 2.4.2.30/Poly(ADP-ribose) Polymerases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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