Document Detail


Antiproliferative effect and ultrastructural alterations induced by psilostachyin on Trypanosoma cruzi.
MedLine Citation:
PMID:  20110908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of psilostachyin, a natural sesquiterpene lactone, on the growth and viability of cultured epimastigotes of Trypanosoma cruzi (Tulahuen) is reported. The antiproliferative effect was evaluated by counting the parasites in a Neubauer chamber and measuring their viability by using the dye exclusion technique. The effect on parasite growth was irreversible at concentrations higher than 1.0 microg/mL and the addition of glutathione only partially blocked the effect of the compound. Moreover, we have studied the effects of this natural compound on parasite ultrastructure by transmission electron microscopy. Interestingly, psilostachyin induced ultrastructural alterations on the parasites at a concentration of 0.5 microg/mL, with important mitochondrial swelling and deformity of the kinetoplast.
Authors:
Valeria Sülsen; Patricia Barrera; Liliana Muschietti; Virginia Martino; Miguel Sosa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-25
Journal Detail:
Title:  Molecules (Basel, Switzerland)     Volume:  15     ISSN:  1420-3049     ISO Abbreviation:  Molecules     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-29     Completed Date:  2010-04-02     Revised Date:  2010-12-15    
Medline Journal Info:
Nlm Unique ID:  100964009     Medline TA:  Molecules     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  545-53     Citation Subset:  IM    
Affiliation:
Cátedra de Farmacognosia, Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, IQUIMEFA (UBA-CONICET), Junín 956 (1113), CABA, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Assay
Cell Death / drug effects
Cell Proliferation / drug effects
Cell Survival / drug effects
Glutathione / pharmacology
Mice
Mice, Inbred BALB C
Parasites / cytology,  drug effects,  growth & development,  ultrastructure
Sesquiterpenes / pharmacology*
Trypanosoma cruzi / cytology*,  drug effects,  growth & development,  ultrastructure*
Chemical
Reg. No./Substance:
0/Sesquiterpenes; 70-18-8/Glutathione

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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