| Antiproliferation effects of ponicidin on human myeloid leukemia cells in vitro. | |
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MedLine Citation:
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PMID: 15756438 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ponicidin, an extract from the Chinese herb Rabdosia rubescens, is currently one of the most important traditional Chinese herbal medicines. Ponicidin has been reported to have anti-tumor effects on a large variety of malignant diseases. In this study, we investigated the anti-proliferation effects of ponicidin on human myeloid K562 and HL-60 cells. Cell viability was measured by MTT assay; cell apoptosis was assessed by flow cytometry, DNA fragmentation analysis and Hoechst 33258 staining. Caspase-3 and poly(ADP-ribose) polymerase (PARP) activation and Bax and Bcl-2 expression were detected by Western blot analysis. The results revealed that ponicidin could significantly inhibit the growth of K562 and HL-60 cells by induction of apoptosis. The suppression was both time- and dose-dependent. Cell apoptosis was observed clearly after the cells were treated with ponicidin for 48-72 h. Western blotting showed cleavage of the caspase-3 zymogen protein (32 kDa) with the appearance of its 17 kDa subunit, together with a cleaved 89-kDa fragment of 116 kDa PARP when apoptosis occurred. Bcl-2 expression was down-regulated while Bax expression up-regulated concurrently when the cells were treated with ponicidin for 24-48 h. Therefore, we conclude that ponicidin has significant anti-proliferation effects by induction of apoptosis on myeloid leukemia cells in vitro, down-regulation of Bcl-2, and up-regulation of Bax, and that activation of caspase-3 and PARP may be an important apoptosis-inducing mechanism. The results suggest that ponicidin may serve as a potential therapeutic agent for leukemia. |
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Authors:
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Jia-Jun Liu; Ren-Wei Huang; Dong-Jun Lin; Xiang-Yuan Wu; Qu Lin; Jun Peng; Xianglin Pan; Yu-Qin Song; Mao-Hong Zhang; Ming Hou; Feng Chen |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oncology reports Volume: 13 ISSN: 1021-335X ISO Abbreviation: Oncol. Rep. Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-03-09 Completed Date: 2005-08-26 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9422756 Medline TA: Oncol Rep Country: Greece |
Other Details:
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Languages: eng Pagination: 653-7 Citation Subset: IM |
Affiliation:
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Sun Yat-sen University, Department of Haematology and Oncology, The Third Affiliated Hospital of Sun Yat-sen University, Tian-He Road 600, Guangzhou, Guangdong 510630, PR China. liujj@gzsums.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology* Apoptosis Bisbenzimidazole / pharmacology Blotting, Western Caspase 3 Caspases / metabolism Cell Proliferation Cell Survival DNA Fragmentation Diterpenes / pharmacology* Dose-Response Relationship, Drug Down-Regulation Drugs, Chinese Herbal Flow Cytometry HL-60 Cells Humans K562 Cells Leukemia, Myeloid / drug therapy*, metabolism* Models, Chemical Plant Extracts / pharmacology Poly(ADP-ribose) Polymerases / metabolism Proto-Oncogene Proteins c-bcl-2 / biosynthesis Time Factors Up-Regulation bcl-2-Associated X Protein |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/BAX protein, human; 0/Diterpenes; 0/Drugs, Chinese Herbal; 0/Plant Extracts; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 23491-44-3/Bisbenzimidazole; 52617-37-5/ponicidin; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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