| Antiproliferation effect and mechanism of prostate cancer cell lines as affected by isoflavones from soybean cake. | |
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MedLine Citation:
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PMID: 19292464 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objectives of this study were to determine the antiproliferation effect of prostate cancer cell lines LNCaP and PC-3 as affected by 4 isoflavone fractions prepared from soybean cake and isoflavone standards genistein and daidzein. With the MTT test, most treatments were effective in inhibiting prostate cancer cell growth at a low dose of 5 and 10 mug/mL. In cell cycle analysis, the fractions of aglycon, a mixture of acetylglucoside and aglycon, as well as genistein and a combination of genistein and daidzein standards exhibited a high G2/M ratio for LNCaP, as did the acetylglucoside, genistein and a combination of genistein and daidzein standards for PC-3. Results of Western blotting revealed an increase in p53 protein expression of LNCaP following treatments of the aglycon fraction, genistein and a combination of genistein and daidzein standards. However, all the treatments did not affect Bcl-2 protein expression significantly in both LNCaP and PC-3 cells. A decline in cyclin B1 expression of LNCaP was observed for all the treatments, with the mixture of acetylglucoside and aglycon possessing the most pronounced effect. But for PC-3, a decrease in cyclin B1 expression was shown for all the isoflavones, with the exception of malonylglucoside, glucoside and acetylglucoside fractions. The outcome of this study may provide a basis for possible production of functional food in the future with soybean cake as raw material. |
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Authors:
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Bai Fu Wang; Jinn Shyan Wang; Jyh Feng Lu; Tsai Hua Kao; Bing Huei Chen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 57 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-03-18 Completed Date: 2009-05-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 2221-32 Citation Subset: IM |
Affiliation:
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Department of Urology, Changhua Christian Hospital, Changhua, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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pharmacology Apoptosis / drug effects, genetics Cell Cycle / drug effects, genetics Cell Division / drug effects* Cell Line, Tumor Gene Expression / drug effects Genes, p53 / genetics Humans Isoflavones / analysis, pharmacology* Male Prostatic Neoplasms / pathology* Soybeans / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Isoflavones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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