Document Detail

Antiproliferation effect and mechanism of prostate cancer cell lines as affected by isoflavones from soybean cake.
MedLine Citation:
PMID:  19292464     Owner:  NLM     Status:  MEDLINE    
The objectives of this study were to determine the antiproliferation effect of prostate cancer cell lines LNCaP and PC-3 as affected by 4 isoflavone fractions prepared from soybean cake and isoflavone standards genistein and daidzein. With the MTT test, most treatments were effective in inhibiting prostate cancer cell growth at a low dose of 5 and 10 mug/mL. In cell cycle analysis, the fractions of aglycon, a mixture of acetylglucoside and aglycon, as well as genistein and a combination of genistein and daidzein standards exhibited a high G2/M ratio for LNCaP, as did the acetylglucoside, genistein and a combination of genistein and daidzein standards for PC-3. Results of Western blotting revealed an increase in p53 protein expression of LNCaP following treatments of the aglycon fraction, genistein and a combination of genistein and daidzein standards. However, all the treatments did not affect Bcl-2 protein expression significantly in both LNCaP and PC-3 cells. A decline in cyclin B1 expression of LNCaP was observed for all the treatments, with the mixture of acetylglucoside and aglycon possessing the most pronounced effect. But for PC-3, a decrease in cyclin B1 expression was shown for all the isoflavones, with the exception of malonylglucoside, glucoside and acetylglucoside fractions. The outcome of this study may provide a basis for possible production of functional food in the future with soybean cake as raw material.
Bai Fu Wang; Jinn Shyan Wang; Jyh Feng Lu; Tsai Hua Kao; Bing Huei Chen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  57     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-18     Completed Date:  2009-05-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2221-32     Citation Subset:  IM    
Department of Urology, Changhua Christian Hospital, Changhua, Taiwan.
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MeSH Terms
Antioxidants / pharmacology
Apoptosis / drug effects,  genetics
Cell Cycle / drug effects,  genetics
Cell Division / drug effects*
Cell Line, Tumor
Gene Expression / drug effects
Genes, p53 / genetics
Isoflavones / analysis,  pharmacology*
Prostatic Neoplasms / pathology*
Soybeans / chemistry*
Reg. No./Substance:
0/Antioxidants; 0/Isoflavones

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