Document Detail

Antiproliferation and apoptosis on RKO colon cancer by Millingtonia hortensis.
MedLine Citation:
PMID:  19083098     Owner:  NLM     Status:  MEDLINE    
Millingtonia hortensis is a medicinal plant widely used in many Asian countries. An aqueous crude extract of this plant has been shown the apoptosis induction on RKO colon cancer cells. However, its mechanism remains unknown. To learn more about this plant extract, we partially purified the crude extract using Sephadex LH-20 and three aqueous fractions were collected. Each fraction was investigated for cytotoxicity using MTT assay. Fraction 1 showed antiproliferative effect on RKO cells with dose-dependent manner, while fraction 2 and 3 had no effect. Induction of apoptosis was determined using flow cytometry and DNA fragmentation method. Apoptotic cell numbers and the appearance of fragmented DNA increased with dose-dependent manner after treatment with fraction 1 for 48 h. We further investigated the expression of apoptotic protein by western blot analysis. Fraction 1 decreased the expression of anti-apoptotic protein, Bcl-xL and p-Bad, while pro-apoptotic protein Bad, was not changed. Fraction 1 also decreased the expression of p-Akt and slightly increased the level of total Akt. These results indicated that fraction 1 is able to inhibit cell proliferation and induce apoptosis on RKO cells by decreasing the expression of Bcl-xL, p-Bad and p-Akt which are involving in survival of cancer cells.
Siwapong Tansuwanwong; Hiroyuki Yamamoto; Kohzoh Imai; Usanee Vinitketkumnuen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant foods for human nutrition (Dordrecht, Netherlands)     Volume:  64     ISSN:  0921-9668     ISO Abbreviation:  Plant Foods Hum Nutr     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-02-09     Completed Date:  2009-05-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8803554     Medline TA:  Plant Foods Hum Nutr     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  11-7     Citation Subset:  IM    
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
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MeSH Terms
Antineoplastic Agents, Phytogenic / pharmacology*
Apoptosis / drug effects*
Apoptosis Regulatory Proteins / genetics,  metabolism
Cell Proliferation / drug effects
Colonic Neoplasms / drug therapy*,  pathology,  prevention & control
DNA Fragmentation / drug effects
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Flavonoids / pharmacology*
Flow Cytometry
Gene Expression Regulation, Neoplastic
Phytotherapy / methods*
Proto-Oncogene Proteins c-akt / genetics,  metabolism
Proto-Oncogene Proteins c-bcl-2 / genetics,  metabolism
Tumor Cells, Cultured
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Apoptosis Regulatory Proteins; 0/Flavonoids; 0/Proto-Oncogene Proteins c-bcl-2; 123442-39-7/hortensin; EC Proteins c-akt

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