| Antiproliferation Effect and Apoptosis Mechanism of Prostate Cancer Cell PC-3 by Flavonoids and Saponins Prepared from Gynostemma pentaphyllum. | |
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MedLine Citation:
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PMID: 21905716 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The objectives of this study were to investigate the antiproliferation and apoptosis mechanism of saponin and flavonoid fractions from Gynostemma pentaphyllum (Thunb.) Makino on prostate cancer cell PC-3. Both flavonoid and saponin fractions were isolated by a column chromatographic method with cosmosil 75C¬18-OPN as adsorbent and elution solvents of ethanol-water (30:70, v/v) for the former and 100% ethanol for the latter, followed by HPLC-MS-MS analysis. Based on MTT assay, the saponin and flavonoid fraction were comparably effective in inhibiting growth of PC-3 cells, with the IC50 being 39.3 and 33.3 g/mL, respectively. Additionally, both fractions induced an arrest of PC-3 cell cycle at both S and G2/M phase, with both early and late apoptotic cell population showing a dose-dependent rise. The western blot assay indicated that the incorporation of flavonoid or saponin fraction could modulate the expression of G2 and M checkpoint regulators, cyclin A and B, as well as the anti-apoptotic proteins Bcl-2 and Bcl-xl, pro-apoptotic proteins Bad and Bax. The expression of the caspase-3 and its activated downstream substrate effectors, DFF45 and poly (ADP-ribose) polymerase-1 (PARP-1) was also increased and followed a dose-dependent manner. All these findings suggest that the apoptosis of PC-3 cells may proceed through the intrinsic mitochondria pathway. |
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Authors:
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Tse-Chou Cheng; Jyh-Feng Lu; Jinn-Shyan Wang; Li-Ju Lin; Hung-I Kuo; Bing Huei Chen |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-11 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: - ISSN: 1520-5118 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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