| Antioxidative capacity of extracts and constituents in Cornus capitata adventitious roots. | |
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MedLine Citation:
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PMID: 13129293 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Radical scavenging activities of extracts and constituents in Cornus capitata adventitious root cultures were evaluated by using 1,1-diphenyl-2-pycrylhydrazyl (DPPH) and superoxide anion radicals. Inhibitory activity against peroxidation of linoleic acid was assayed by using the thiobarbituric acid (TBA) method. Ethyl acetate and aqueous fractions were prepared from adventitious roots cultured in Murashige-Skoog liquid medium with 0.1 microM Cu(2+) (0.1CuMS) or 10 microM Cu(2+) (10CuMS). The highest scavenging activities on DPPH and superoxide anion radicals were observed in the ethyl acetate fraction from 0.1CuMS. In the inhibitory activity against linoleic acid oxidation, the ethyl acetate fraction from 10CuMS was highest among the fractions tested. The ethyl acetate fraction of adventitious roots cultured in 0.1CuMS contained mainly galloylglucoses (1,2,3,6-tetragalloylglucose and 1,2,3,4,6-pentagalloylglucose). The ethyl acetate fraction of adventitious roots cultured in 10CuMS contained mainly ellagic acid derivatives [3,3'-di-O-methylellagic acid 4-(5"-acetyl)-alpha-L-arabinofuranoside and stenophyllin H1]. Aqueous fractions prepared from both media contained iridoid glycosides (dihydrocornin and cornin). Tetra- and pentagalloylglucoses showed strong inhibitory activities (61.9 and 85.2%, respectively) against linoleic acid oxidation relative to those of butylated hydroxytoluene (BHT) (91.1%) or alpha-tocopherol (49.5%) at 50 microM concentration. Although both ellagic acid derivatives had weak activities (<50%) on DPPH and superoxide anion radical scavenging, 3,3'-di-O-methylellagic acid 4-(5"-acetyl)-alpha-L-arabinofuranoside was stronger (74.7%) than alpha-tocopherol (49.5%) in inhibiting linoleic acid oxidation at 50 microM concentration. Iridoid glycosides exhibited little activity against DPPH and superoxide anion radicals or against oxidation of linoleic acid. |
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Authors:
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Norie Tanaka; Kazutaka Nishikawa; Kanji Ishimaru |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 51 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2003 Sep |
Date Detail:
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Created Date: 2003-09-17 Completed Date: 2003-10-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 5906-10 Citation Subset: IM |
Affiliation:
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Venture Business Laboratory and Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, 1 Honjo, Saga 840-8502, Japan. nishikawn@tri.pref.osaka.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates Antioxidants / analysis*, pharmacology Biphenyl Compounds Cornus / chemistry* Free Radical Scavengers / analysis Linoleic Acid / chemistry Lipid Peroxidation / drug effects Picrates / chemistry Plant Extracts / analysis*, pharmacology Plant Roots / chemistry* Superoxides / chemistry Thiobarbituric Acid Reactive Substances |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Antioxidants; 0/Biphenyl Compounds; 0/Free Radical Scavengers; 0/Picrates; 0/Plant Extracts; 0/Thiobarbituric Acid Reactive Substances; 11062-77-4/Superoxides; 141-78-6/ethyl acetate; 1898-66-4/2,2-diphenyl-1-picrylhydrazyl; 2197-37-7/Linoleic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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