Document Detail


Antioxidative capacity of extracts and constituents in Cornus capitata adventitious roots.
MedLine Citation:
PMID:  13129293     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Radical scavenging activities of extracts and constituents in Cornus capitata adventitious root cultures were evaluated by using 1,1-diphenyl-2-pycrylhydrazyl (DPPH) and superoxide anion radicals. Inhibitory activity against peroxidation of linoleic acid was assayed by using the thiobarbituric acid (TBA) method. Ethyl acetate and aqueous fractions were prepared from adventitious roots cultured in Murashige-Skoog liquid medium with 0.1 microM Cu(2+) (0.1CuMS) or 10 microM Cu(2+) (10CuMS). The highest scavenging activities on DPPH and superoxide anion radicals were observed in the ethyl acetate fraction from 0.1CuMS. In the inhibitory activity against linoleic acid oxidation, the ethyl acetate fraction from 10CuMS was highest among the fractions tested. The ethyl acetate fraction of adventitious roots cultured in 0.1CuMS contained mainly galloylglucoses (1,2,3,6-tetragalloylglucose and 1,2,3,4,6-pentagalloylglucose). The ethyl acetate fraction of adventitious roots cultured in 10CuMS contained mainly ellagic acid derivatives [3,3'-di-O-methylellagic acid 4-(5"-acetyl)-alpha-L-arabinofuranoside and stenophyllin H1]. Aqueous fractions prepared from both media contained iridoid glycosides (dihydrocornin and cornin). Tetra- and pentagalloylglucoses showed strong inhibitory activities (61.9 and 85.2%, respectively) against linoleic acid oxidation relative to those of butylated hydroxytoluene (BHT) (91.1%) or alpha-tocopherol (49.5%) at 50 microM concentration. Although both ellagic acid derivatives had weak activities (<50%) on DPPH and superoxide anion radical scavenging, 3,3'-di-O-methylellagic acid 4-(5"-acetyl)-alpha-L-arabinofuranoside was stronger (74.7%) than alpha-tocopherol (49.5%) in inhibiting linoleic acid oxidation at 50 microM concentration. Iridoid glycosides exhibited little activity against DPPH and superoxide anion radicals or against oxidation of linoleic acid.
Authors:
Norie Tanaka; Kazutaka Nishikawa; Kanji Ishimaru
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  51     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-09-17     Completed Date:  2003-10-27     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5906-10     Citation Subset:  IM    
Affiliation:
Venture Business Laboratory and Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, 1 Honjo, Saga 840-8502, Japan. nishikawn@tri.pref.osaka.jp
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MeSH Terms
Descriptor/Qualifier:
Acetates
Antioxidants / analysis*,  pharmacology
Biphenyl Compounds
Cornus / chemistry*
Free Radical Scavengers / analysis
Linoleic Acid / chemistry
Lipid Peroxidation / drug effects
Picrates / chemistry
Plant Extracts / analysis*,  pharmacology
Plant Roots / chemistry*
Superoxides / chemistry
Thiobarbituric Acid Reactive Substances
Chemical
Reg. No./Substance:
0/Acetates; 0/Antioxidants; 0/Biphenyl Compounds; 0/Free Radical Scavengers; 0/Picrates; 0/Plant Extracts; 0/Thiobarbituric Acid Reactive Substances; 11062-77-4/Superoxides; 141-78-6/ethyl acetate; 1898-66-4/2,2-diphenyl-1-picrylhydrazyl; 2197-37-7/Linoleic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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