Document Detail

Antioxidative activity of amino acids on tissue oxidative stress in human intestinal epithelial cell model.
MedLine Citation:
PMID:  17883254     Owner:  NLM     Status:  MEDLINE    
The protective effects of amino acids against H 2O 2-induced oxidative stress were investigated in an in vitro assay using human intestinal epithelial cells. Caco-2 cells were pretreated with amino acids (1, 2, and 5 mM) for 2 h and then stimulated with 1 mM H 2O 2 for 6 h. The secretion of IL-8, a proinflammatory mediator, was determined by ELISA as an indicator of tissue oxidative stress. The inhibition of H 2O 2-induced IL-8 secretion from Caco-2 cells was observed by pretreatment with Cys, Val, Ile, Leu, Trp, His, Lys, and Ala. Cys enhanced glutathione (GSH) biosynthesis enzyme activity and increased cellular GSH levels. Branched-chain amino acids such as Val, Ile, and Leu elevated activities of GSH S-transferase (GST) and catalase. Trp, His, and Lys caused increases in GST activity. Ala enhanced GSH reductase activity. These data suggest that specific amino acids exert protective effects against tissue oxidative stress in intestinal epithelial cells based on the structure.
Shigeru Katayama; Yoshinori Mine
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-09-21
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  55     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-12     Completed Date:  2007-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8458-64     Citation Subset:  IM    
Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
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MeSH Terms
Amino Acids / pharmacology*
Antioxidants / pharmacology*
Caco-2 Cells
Glutathione / analysis
Hydrogen Peroxide / pharmacology
Interleukin-8 / secretion
Intestinal Mucosa / cytology,  drug effects*,  metabolism
Models, Biological
Oxidative Stress / drug effects*
Reg. No./Substance:
0/Amino Acids; 0/Antioxidants; 0/Interleukin-8; 70-18-8/Glutathione; 7722-84-1/Hydrogen Peroxide

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