| Antioxidative activity of amino acids on tissue oxidative stress in human intestinal epithelial cell model. | |
| | |
MedLine Citation:
|
PMID: 17883254 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The protective effects of amino acids against H 2O 2-induced oxidative stress were investigated in an in vitro assay using human intestinal epithelial cells. Caco-2 cells were pretreated with amino acids (1, 2, and 5 mM) for 2 h and then stimulated with 1 mM H 2O 2 for 6 h. The secretion of IL-8, a proinflammatory mediator, was determined by ELISA as an indicator of tissue oxidative stress. The inhibition of H 2O 2-induced IL-8 secretion from Caco-2 cells was observed by pretreatment with Cys, Val, Ile, Leu, Trp, His, Lys, and Ala. Cys enhanced glutathione (GSH) biosynthesis enzyme activity and increased cellular GSH levels. Branched-chain amino acids such as Val, Ile, and Leu elevated activities of GSH S-transferase (GST) and catalase. Trp, His, and Lys caused increases in GST activity. Ala enhanced GSH reductase activity. These data suggest that specific amino acids exert protective effects against tissue oxidative stress in intestinal epithelial cells based on the structure. |
| | |
Authors:
|
Shigeru Katayama; Yoshinori Mine |
Related Documents
:
|
7485014 - Modulatory impact of acid and pepsin on esophageal hydrophobicity in humans. 7638734 - Mechanisms of gastric acid secretion in the fetal rabbit. 17996964 - Cyclopamine inhibition of the sonic hedgehog pathway in the stomach requires concomitan... 2891584 - Regulation of gastric somatostatin secretion in the mouse by luminal acidity: a local f... 922754 - Cellular response to treatment with 4-(3-(2-chloroethyl)-3-nitrosoureido)-cis-cyclohexa... 17389774 - Antihyaluronidase action of ellagic acid effectively prevents polyspermy as a result of... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-09-21 |
Journal Detail:
|
Title: Journal of agricultural and food chemistry Volume: 55 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2007 Oct |
Date Detail:
|
Created Date: 2007-10-12 Completed Date: 2007-11-26 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 8458-64 Citation Subset: IM |
Affiliation:
|
Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada. ymine@uoguelph.ca |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
pharmacology* Antioxidants / pharmacology* Caco-2 Cells Glutathione / analysis Humans Hydrogen Peroxide / pharmacology Interleukin-8 / secretion Intestinal Mucosa / cytology, drug effects*, metabolism Models, Biological Oxidative Stress / drug effects* |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Antioxidants; 0/Interleukin-8; 70-18-8/Glutathione; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Optimization of multiple headspace solid-phase microextraction for the quantification of volatile co...
Next Document: Peanut allergen Ara h 1 interacts with proanthocyanidins into higher molecular weight complexes.