Document Detail

Antioxidative activity of 3,4-dihydroxyphenylacetic acid and caffeic acid in rat plasma.
MedLine Citation:
PMID:  11768155     Owner:  NLM     Status:  MEDLINE    
The purpose of the present paper is to study and compare in vitro the inhibitory effect of 3,4-dihydroxyphenylacetic acid (DOPAC) and caffeic acid (CA) on lipid peroxidation in rat plasma. Rat plasma was oxidized at 37 degrees C by the radical initiators 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) or 2,2'-azobis(4-methoxy-2,4-dimethylvaleronitrile) (MeO-AMVN). The consumption of endogenous alpha-tocopherol (alpha-TOH) and the accumulation of conjugated diene hydroperoxides were measured by high-performance liquid chromatography and by ultraviolet spectroscopy, respectively. Alpha-TOH was consumed at the same rate in the presence of 20 mM AAPH or 2 mM MeO-AMVN. DOPAC and CA suppressed the alpha-TOH consumption in a dose-dependent manner. A concentration of 50 microM of both phenolic acids was sufficient to induce a lag phase and to delay the rate of alpha-TOH consumption. The effect was more pronounced in rat plasma oxidation by AAPH than by MeO-AMVN. CA spared vitamin E more effectively than DOPAC in both oxidations. DOPAC and CA suppressed the formation of conjugated diene hydroperoxides. DOPAC and CA at concentration 50 microM suppressed alpha-TOH consumption during oxidation of soybean phosphatidylcholine (2.8 mM) multilamellar vesicles containing 15 microM alpha-TOH, in which the lipophilic initiator 2,2'-azobis (2,4-dimethylvaleronitrile) (6 mM) was incorporated. In conclusion, we demonstrated that DOPAC and CA in micromolar concentrations have antioxidant activity in rat plasma, a medium very close to the conditions in vivo, suggesting that supplementation with the phenolic acids will provide significant antioxidant protection.
V Raneva; H Shimasaki; Y Ishida; N Ueta; E Niki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  36     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-12-20     Completed Date:  2002-05-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1111-6     Citation Subset:  IM    
First Department of Biochemistry, Teikyo University School of Medicine, Tokyo, Japan.
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MeSH Terms
3,4-Dihydroxyphenylacetic Acid / blood,  pharmacology*
Amidines / pharmacology
Antioxidants / pharmacology*
Azo Compounds / pharmacology
Caffeic Acids / blood,  pharmacology*
Edetic Acid / pharmacology
Lipid Peroxidation / drug effects*
Lipids / blood
Liposomes / chemistry
Methanol / pharmacology
Nitriles / pharmacology
Rats, Sprague-Dawley
alpha-Tocopherol / blood
Reg. No./Substance:
0/Amidines; 0/Antioxidants; 0/Azo Compounds; 0/Caffeic Acids; 0/Lipids; 0/Liposomes; 0/Nitriles; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 13217-66-8/2,2'-azobis(2-amidinopropane); 15545-97-8/V 70; 331-39-5/caffeic acid; 59-02-9/alpha-Tocopherol; 60-00-4/Edetic Acid; 67-56-1/Methanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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