| Antioxidative activity of 3,4-dihydroxyphenylacetic acid and caffeic acid in rat plasma. | |
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MedLine Citation:
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PMID: 11768155 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of the present paper is to study and compare in vitro the inhibitory effect of 3,4-dihydroxyphenylacetic acid (DOPAC) and caffeic acid (CA) on lipid peroxidation in rat plasma. Rat plasma was oxidized at 37 degrees C by the radical initiators 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) or 2,2'-azobis(4-methoxy-2,4-dimethylvaleronitrile) (MeO-AMVN). The consumption of endogenous alpha-tocopherol (alpha-TOH) and the accumulation of conjugated diene hydroperoxides were measured by high-performance liquid chromatography and by ultraviolet spectroscopy, respectively. Alpha-TOH was consumed at the same rate in the presence of 20 mM AAPH or 2 mM MeO-AMVN. DOPAC and CA suppressed the alpha-TOH consumption in a dose-dependent manner. A concentration of 50 microM of both phenolic acids was sufficient to induce a lag phase and to delay the rate of alpha-TOH consumption. The effect was more pronounced in rat plasma oxidation by AAPH than by MeO-AMVN. CA spared vitamin E more effectively than DOPAC in both oxidations. DOPAC and CA suppressed the formation of conjugated diene hydroperoxides. DOPAC and CA at concentration 50 microM suppressed alpha-TOH consumption during oxidation of soybean phosphatidylcholine (2.8 mM) multilamellar vesicles containing 15 microM alpha-TOH, in which the lipophilic initiator 2,2'-azobis (2,4-dimethylvaleronitrile) (6 mM) was incorporated. In conclusion, we demonstrated that DOPAC and CA in micromolar concentrations have antioxidant activity in rat plasma, a medium very close to the conditions in vivo, suggesting that supplementation with the phenolic acids will provide significant antioxidant protection. |
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Authors:
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V Raneva; H Shimasaki; Y Ishida; N Ueta; E Niki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Lipids Volume: 36 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 2001 Oct |
Date Detail:
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Created Date: 2001-12-20 Completed Date: 2002-05-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: United States |
Other Details:
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Languages: eng Pagination: 1111-6 Citation Subset: IM |
Affiliation:
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First Department of Biochemistry, Teikyo University School of Medicine, Tokyo, Japan. villy@med.teikyo-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3,4-Dihydroxyphenylacetic Acid
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blood,
pharmacology* Amidines / pharmacology Animals Antioxidants / pharmacology* Azo Compounds / pharmacology Caffeic Acids / blood, pharmacology* Edetic Acid / pharmacology Kinetics Lipid Peroxidation / drug effects* Lipids / blood Liposomes / chemistry Male Methanol / pharmacology Nitriles / pharmacology Oxidation-Reduction Rats Rats, Sprague-Dawley alpha-Tocopherol / blood |
| Chemical | |
Reg. No./Substance:
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0/Amidines; 0/Antioxidants; 0/Azo Compounds; 0/Caffeic Acids; 0/Lipids; 0/Liposomes; 0/Nitriles; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 13217-66-8/2,2'-azobis(2-amidinopropane); 15545-97-8/V 70; 331-39-5/caffeic acid; 59-02-9/alpha-Tocopherol; 60-00-4/Edetic Acid; 67-56-1/Methanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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