Document Detail

Antioxidative action of royal jelly in the yeast cell.
MedLine Citation:
PMID:  17383134     Owner:  NLM     Status:  MEDLINE    
Royal jelly is a bee product, secreted from the hypopharingeal and mandibular glands of worker bees. There are many reports on pharmacological activities of royal jelly in experimental animals, but there are few about its antioxidative properties connected to aging. The aim of the work was to investigate the antioxidative action of royal jelly in the cell of the yeast Saccharomyces cerevisiae as a model organism. Yeast was cultivated in YEPD medium enriched with different concentrations of royal jelly like 1, 2 and 5 g/L. Yeast growth was monitored by measuring optical density. At different time points cell energy metabolic activity was measured using the cell energy metabolism indicator resazurin, and 2',7'-dichlorofluorescein was applied to estimate intracellular oxidation. Additionally, protein profile of cell extract was analyzed by 2-D electrophoresis. Results showed that royal jelly decreased intracellular oxidation in a dose dependent manner. Additionally it affected growth and cell energy metabolic activity in a growth phase dependent manner. Protein profile analysis showed that royal jelly in the cell does not act only as a scavenger of reactive oxygen species, but it also affects protein expression. Differentially expressed proteins were identified.
Polona Jamnik; Dusan Goranovic; Peter Raspor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-20
Journal Detail:
Title:  Experimental gerontology     Volume:  42     ISSN:  0531-5565     ISO Abbreviation:  Exp. Gerontol.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-05-22     Completed Date:  2007-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0047061     Medline TA:  Exp Gerontol     Country:  England    
Other Details:
Languages:  eng     Pagination:  594-600     Citation Subset:  IM    
University of Ljubljana, Biotechnical Faculty, Food Science and Technology Department, Chair of Biotechnology, Jamnikarjeva 101, Ljubljana, Slovenia.
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MeSH Terms
Antioxidants / pharmacology*
Cell Division / drug effects
Energy Metabolism / drug effects
Fatty Acids / pharmacology*
Insect Hormones / pharmacology
Saccharomyces cerevisiae / drug effects,  metabolism,  physiology*
Saccharomyces cerevisiae Proteins / drug effects,  metabolism
Reg. No./Substance:
0/Antioxidants; 0/Fatty Acids; 0/Insect Hormones; 0/Saccharomyces cerevisiae Proteins; 8031-67-2/royal jelly

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