Document Detail

Antioxidants rescue stressed embryos at a rate comparable with co-culturing of embryos with human umbilical cord mesenchymal cells.
MedLine Citation:
PMID:  21207131     Owner:  NLM     Status:  MEDLINE    
PURPOSE: During laboratory manipulations, oocytes and embryos are inevitably exposed to suboptimal conditions that interfere with the normal development of embryos.
MATERIALS AND METHODS: In this study, we examined the effects of antioxidants, feeder cells and a conditioned medium on embryo development and cleavage rate following exposure of the embryos to suboptimal conditions. We exposed mouse two-cell embryos to visible light and divided them into four groups: control (E-ctr), co-culture (Co-c), conditioned medium (Cndm) and antioxidant-plus medium (Aopm). We used human umbilical cord matrix-derived mesenchymal cells for co-culture. A group of embryos was not exposed to visible light and served as the non-exposed control (NE-ctr) group.
RESULTS: The developmental rate was higher in NE-ctr embryos than in the E-ctr group. Exposed embryos in the various groups showed a comparable developmental rate at different stages. Blastomere number significantly increased (P < 0.05) in the Co-c and Aopm groups compared with the E-ctr and Cndm groups. No significant difference was observed between the Co-c and Aopm groups.
CONCLUSIONS: Our data indicate that in suboptimal conditions, antioxidants could improve the embryo cleavage rate in the same way as feeder cells. Antioxidants probably improve embryo quality through their ability to scavenge reactive oxygen species.
Ghazaleh Moshkdanian; Seyed Noureddin Nematollahi-Mahani; Fatemeh Pouya; Amirmahdi Nematollahi-Mahani
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2011-01-05
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  28     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-06-15     Completed Date:  2011-10-17     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  343-9     Citation Subset:  IM    
Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Science, Kerman, Iran.
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MeSH Terms
Antioxidants / pharmacology*
Blastocyst / drug effects
Cell Differentiation / drug effects
Cells, Cultured
Cleavage Stage, Ovum / drug effects
Coculture Techniques / methods*
Culture Media, Conditioned
Embryonic Development / drug effects
Mesenchymal Stromal Cells / cytology*,  metabolism
Reactive Oxygen Species / metabolism
Umbilical Cord / cytology*
Reg. No./Substance:
0/Antioxidants; 0/Culture Media, Conditioned; 0/Reactive Oxygen Species

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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