Document Detail


Antioxidants modulate the antiproliferative effects of nitric oxide on vascular smooth muscle cells and adventitial fibroblasts by regulating oxidative stress.
MedLine Citation:
PMID:  21944289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: S-nitrosothiols (SNO) release nitric oxide (NO) through interaction with ascorbic acid (AA). However, little is known about their combined effect in the vasculature. The aim of this study was to investigate the effect of AA on SNO-mediated NO release, proliferation, cell cycle progression, cell death, and oxidative stress in vascular cells.
METHODS: Vascular smooth muscle cells and adventitial fibroblasts harvested from the aortae of Sprague-Dawley rats were treated with AA, ± S-nitrosoglutathione (GSNO), or ± diethylenetriamine NONOate (DETA/NO). NO release, proliferation, cell cycle progression, cell death, and oxidative stress were determined by the Griess reaction, [(3)H]-thymidine incorporation, flow cytometry, trypan blue exclusion, and 5-(and-6)chloromethyl-2',7'dichlorodihydrofluorescein staining, respectively.
RESULTS: AA increased NO release from GSNO 3-fold (P < .001). GSNO and DETA/NO significantly decreased proliferation, but AA abrogated this effect (P < .05). Mirroring the proliferation data, changes in cell cycle progression induced by GSNO and DETA/NO were reversed by the addition of AA. GSNO- and DETA/NO-mediated increases in oxidative stress were significantly decreased by the addition of AA (P < .001).
CONCLUSIONS: Despite causing increased NO release from GSNO, AA reduced the antiproliferative and cell cycle effects of GSNO and DETA/NO through the modulation of oxidative stress.
Authors:
Elaine K Gregory; Ashley K Vavra; Edward S Moreira; George E Havelka; Qun Jiang; Vanessa R Lee; Robert Van Lith; Guillermo A Ameer; Melina R Kibbe
Related Documents :
19285749 - Osteopontin is involved in the development of acquired chemo-resistance of cisplatin in...
21484789 - Nuclear her3 is associated with favourable overall survival in uveal melanoma.
7575589 - Generation of resistance to the diphenyl ether herbicide acifluorfen by mel cells.
19943199 - Autophagy inhibition in combination cancer treatment.
8019989 - The development of drug resistance by tumor cells in vitro is accompanied by the develo...
9398059 - Characterisation of novel human lung carcinoma cell lines selected for resistance to an...
3817809 - Posttreatment with sodium arsenite is coclastogenic in log phase but not in stationary ...
25469689 - Effects of synthetic biomacromolecule addition on the flow behaviour of concentrated me...
8667989 - Regulation of transforming growth factor-beta 1-mediated apoptosis in head and neck squ...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-09-23
Journal Detail:
Title:  American journal of surgery     Volume:  202     ISSN:  1879-1883     ISO Abbreviation:  Am. J. Surg.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-10-21     Completed Date:  2011-12-29     Revised Date:  2014-09-05    
Medline Journal Info:
Nlm Unique ID:  0370473     Medline TA:  Am J Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  536-40     Citation Subset:  AIM; IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / pharmacology*
Ascorbic Acid / pharmacology
Cell Cycle / drug effects
Cell Proliferation / drug effects
Cells, Cultured
Connective Tissue
Fibroblasts / drug effects*,  physiology
Muscle, Smooth, Vascular / cytology
Myocytes, Smooth Muscle / drug effects*,  physiology
Nitric Oxide / metabolism*
Nitric Oxide Donors / pharmacology
Nitroso Compounds / pharmacology
Oxidative Stress / drug effects*
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species
S-Nitrosoglutathione / pharmacology
Grant Support
ID/Acronym/Agency:
K08 HL084203/HL/NHLBI NIH HHS; K08 HL084203-05/HL/NHLBI NIH HHS; K08HL084203/HL/NHLBI NIH HHS; T32 HL094293-01/HL/NHLBI NIH HHS; T32 HL094293-03/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Nitric Oxide Donors; 0/Nitroso Compounds; 0/Reactive Oxygen Species; 146724-94-9/2,2'-(hydroxynitrosohydrazono)bis-ethanamine; 31C4KY9ESH/Nitric Oxide; 57564-91-7/S-Nitrosoglutathione; PQ6CK8PD0R/Ascorbic Acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Variable effects of dipteran parasitoids and management treatment on grasshopper fecundity in a tall...
Next Document:  Veterans Affairs general surgery service: the last bastion of integrated specialty care.