| Antioxidants modulate the antiproliferative effects of nitric oxide on vascular smooth muscle cells and adventitial fibroblasts by regulating oxidative stress. | |
| | |
MedLine Citation:
|
PMID: 21944289 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: S-nitrosothiols (SNO) release nitric oxide (NO) through interaction with ascorbic acid (AA). However, little is known about their combined effect in the vasculature. The aim of this study was to investigate the effect of AA on SNO-mediated NO release, proliferation, cell cycle progression, cell death, and oxidative stress in vascular cells. METHODS: Vascular smooth muscle cells and adventitial fibroblasts harvested from the aortae of Sprague-Dawley rats were treated with AA, ± S-nitrosoglutathione (GSNO), or ± diethylenetriamine NONOate (DETA/NO). NO release, proliferation, cell cycle progression, cell death, and oxidative stress were determined by the Griess reaction, [(3)H]-thymidine incorporation, flow cytometry, trypan blue exclusion, and 5-(and-6)chloromethyl-2',7'dichlorodihydrofluorescein staining, respectively. RESULTS: AA increased NO release from GSNO 3-fold (P < .001). GSNO and DETA/NO significantly decreased proliferation, but AA abrogated this effect (P < .05). Mirroring the proliferation data, changes in cell cycle progression induced by GSNO and DETA/NO were reversed by the addition of AA. GSNO- and DETA/NO-mediated increases in oxidative stress were significantly decreased by the addition of AA (P < .001). CONCLUSIONS: Despite causing increased NO release from GSNO, AA reduced the antiproliferative and cell cycle effects of GSNO and DETA/NO through the modulation of oxidative stress. |
| | |
Authors:
|
Elaine K Gregory; Ashley K Vavra; Edward S Moreira; George E Havelka; Qun Jiang; Vanessa R Lee; Robert Van Lith; Guillermo A Ameer; Melina R Kibbe |
Related Documents
:
|
152739 - Differentiation of mouse myeloid leukemia cells is inhibited by a factor from non-diffe... 16505119 - The inverse relationship between reduced folate carrier function and pemetrexed activit... 19528459 - 14-3-3sigma-dependent resistance to cisplatin. 9219509 - Resistance to paclitaxel mediated by p-glycoprotein can be modulated by changes in the ... 22203419 - Hydrogen sulfide protects h9c2 cells against doxorubicin-induced cardiotoxicity through... 3593779 - Studies on the mechanism of selective retention of porphyrins and metalloporphyrins by ... 1385879 - Alterations of skin-associated lymphoid tissue in the carcinogenesis of arsenical skin ... 18522489 - Redox regulation of cell survival. 12093849 - Trophectoderm growth and bilateral symmetry of the blastocyst in the mouse. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-09-23 |
Journal Detail:
|
Title: American journal of surgery Volume: 202 ISSN: 1879-1883 ISO Abbreviation: Am. J. Surg. Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-10-21 Completed Date: 2011-12-29 Revised Date: 2013-02-19 |
Medline Journal Info:
|
Nlm Unique ID: 0370473 Medline TA: Am J Surg Country: United States |
Other Details:
|
Languages: eng Pagination: 536-40 Citation Subset: AIM; IM |
Copyright Information:
|
Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
|
Division of Vascular Surgery, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antioxidants / pharmacology* Ascorbic Acid / pharmacology Cell Cycle / drug effects Cell Proliferation / drug effects Cells, Cultured Connective Tissue Fibroblasts / drug effects*, physiology Muscle, Smooth, Vascular / cytology Myocytes, Smooth Muscle / drug effects*, physiology Nitric Oxide / metabolism* Nitric Oxide Donors / pharmacology Nitroso Compounds / pharmacology Oxidative Stress / drug effects* Rats Rats, Sprague-Dawley Reactive Oxygen Species S-Nitrosoglutathione / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
K08 HL084203-05/HL/NHLBI NIH HHS; K08HL084203/HL/NHLBI NIH HHS; T32 HL094293-01/HL/NHLBI NIH HHS; T32 HL094293-03/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 0/Nitric Oxide Donors; 0/Nitroso Compounds; 0/Reactive Oxygen Species; 10102-43-9/Nitric Oxide; 146724-94-9/2,2'-(hydroxynitrosohydrazono)bis-ethanamine; 50-81-7/Ascorbic Acid; 57564-91-7/S-Nitrosoglutathione |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Variable effects of dipteran parasitoids and management treatment on grasshopper fecundity in a tall...
Next Document: Veterans Affairs general surgery service: the last bastion of integrated specialty care.