| Antioxidants attenuate nuclear factor-kappa B activation and tumor necrosis factor-alpha production in alcoholic hepatitis patient monocytes and rat Kupffer cells, in vitro. | |
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MedLine Citation:
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PMID: 10614719 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There is increased tumor necrosis factor-alpha (TNF) activity in alcoholic hepatitis (AH). OBJECTIVES: To examine the effects of antioxidants and glutathione enhancing agents on NF-kappaB activation and TNF production in Kupffer cells and monocytes. DESIGN AND METHODS: Isolated rat Kupffer cells and peripheral blood monocytes from AH patients were treated in vitro. NF-kappaB activation was assessed by electrophoretic mobility shift assay and TNF was measured in cell culture supernatants. RESULTS: Monocytes from AH patients had greater TNF production compared to normal volunteers. Pretreatment with antioxidants or gluathione enhancing agents inhibited TNF production and NF-kappaB activation in both monocytes from normal and AH patients as well as in rat Kupffer cells. CONCLUSIONS: There may be a therapeutic role for antioxidants or glutathione enhancing agents in disease states with increased TNF activity such as AH. |
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Authors:
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D B Hill; R Devalaraja; S Joshi-Barve; S Barve; C J McClain |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Clinical biochemistry Volume: 32 ISSN: 0009-9120 ISO Abbreviation: Clin. Biochem. Publication Date: 1999 Oct |
Date Detail:
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Created Date: 2000-01-13 Completed Date: 2000-01-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0133660 Medline TA: Clin Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 563-70 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, University of Kentucky Medical Center, Lexington 40536-0084, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / pharmacology* Glutathione / metabolism Hepatitis, Alcoholic / blood, metabolism* Humans Kupffer Cells / drug effects*, metabolism Male Monocytes / drug effects*, metabolism NF-kappa B / metabolism* Rats Rats, Sprague-Dawley Tumor Necrosis Factor-alpha / biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
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1K20AA00190/AA/NIAAA NIH HHS; K211AA00205-02/AA/NIAAA NIH HHS; R01AA010762/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/NF-kappa B; 0/Tumor Necrosis Factor-alpha; 70-18-8/Glutathione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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