Document Detail

Antioxidant supplementation and subsequent oxidative stress of horses during an 80-km endurance race.
MedLine Citation:
PMID:  14974559     Owner:  NLM     Status:  MEDLINE    
This study tested the development of oxidative stress and the effects of antioxidant supplementation in an 80-km ride. A precompetition survey revealed that no competitor would participate without vitamin E supplementation; therefore, 46 horses were paired for past performances and randomly assigned to two groups of 23 each for 3 wk of supplementation before the ride. One group (E) was orally supplemented with 5,000 IU of vitamin E per day; the other group (E+C) received that dose of vitamin E plus 7 g/d of vitamin C. Blood samples, temperature, and heart rate were taken the day before the race, at 21 and 56 km during the ride, at completion, and after 20 min of recovery. Plasma was assayed for lipid hydroperoxides, alpha-tocopherol, total ascorbate, albumin, creatine kinase (CK), and aspartate aminotransferase (AST). Total glutathione and glutathione peroxidase activity were determined in red blood cells and white blood cells. Thirty-four horses completed the race, 12 horses (six in E and six in E+C) did not finish for reasons including lameness, metabolic problems, and rider option. Plasma ascorbate was higher (P = 0.045) in the E+C group than in the E group. Other than ascorbate, neither antioxidant status nor CK and AST activities were affected by supplementation with E+C vs. E. Red blood cell glutathione peroxidase, white blood cell total glutathione, lipid hydroperoxides, CK, and AST increased, and red blood cell total glutathione and white blood cell glutathione peroxidase activity decreased with distance (P < 0.001). Positive correlations were found for plasma lipid hydroperoxides on CK (r = 0.25; P = 0.001) and AST (r = 0.33; P < 0.001). These results establish an association between muscle leakage and a cumulative index of oxidative stress.
C A Williams; D S Kronfeldt; T M Hess; K E Saker; J N Waldron; K M Crandell; R M Hoffman; P A Harris
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of animal science     Volume:  82     ISSN:  0021-8812     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-02-20     Completed Date:  2004-08-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  588-94     Citation Subset:  IM    
Department of Animal Science, Rutgers-The State University of New Jersey, Cook College, New Brunswick 08901, USA.
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MeSH Terms
Animal Welfare
Antioxidants / administration & dosage,  pharmacology*
Ascorbic Acid / administration & dosage,  pharmacology
Aspartate Aminotransferases / metabolism
Creatine Kinase / metabolism
Dietary Supplements
Erythrocytes / enzymology,  metabolism
Glutathione / metabolism
Glutathione Peroxidase / metabolism
Heart Rate / drug effects
Horses / physiology*
Lipid Peroxidation / drug effects
Muscle, Skeletal / drug effects,  enzymology,  metabolism*
Oxidative Stress / drug effects*
Physical Endurance / drug effects*,  physiology
Random Allocation
Running / physiology
Vitamin E / administration & dosage,  pharmacology
Reg. No./Substance:
0/Antioxidants; 1406-18-4/Vitamin E; 50-81-7/Ascorbic Acid; 70-18-8/Glutathione; EC Peroxidase; EC Aminotransferases; EC Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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