Document Detail


Antioxidant reactivity toward nitroxide probes anchored into human serum albumin. A new model for studying antioxidant repairing capacity of protein radicals.
MedLine Citation:
PMID:  19804973     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new strategy to evaluate accessibility of antioxidants to radical proteins has been developed using nitroxide prefluorescent probes anchored into human serum albumin (HSA). Binding association constants for the nitroxide probes C(343)T and QT with HSA were 5 x 10(4) and 9 x 10(4)M(-1), respectively. Rate constants for the nitroxide reduction by antioxidants in HSA were determined finding k(HSA)/k(buffer) ratio of 0.8, 1.9, and 0.075 for ascorbic acid, Trolox, and caffeic acid, respectively, for the nitroxide C(343)T reduction.
Authors:
Alexis Asp??e; Alejandra Orrego; Emilio Alarc??n; Camilo L??pez-Alarc??n; Horacio Poblete; Danilo Gonz??lez-Nilo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-22
Journal Detail:
Title:  Bioorganic & medicinal chemistry letters     Volume:  19     ISSN:  1464-3405     ISO Abbreviation:  Bioorg. Med. Chem. Lett.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-26     Completed Date:  2010-01-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107377     Medline TA:  Bioorg Med Chem Lett     Country:  England    
Other Details:
Languages:  eng     Pagination:  6382-5     Citation Subset:  IM    
Affiliation:
Facultad de Qu??mica y Biolog??a, Universidad de Santiago de Chile, Casilla 40 Correo 33, Santiago, Chile.
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MeSH Terms
Descriptor/Qualifier:
Albumins / physiology*
Anaerobiosis / physiology
Antioxidants / metabolism*
Ascorbic Acid / metabolism*
Caffeic Acids / pharmacology*
Humans
Nitric Oxide / metabolism
Serum Albumin / drug effects,  metabolism*
Thermodynamics
Chemical
Reg. No./Substance:
0/Albumins; 0/Antioxidants; 0/Caffeic Acids; 0/Serum Albumin; 10102-43-9/Nitric Oxide; 331-39-5/caffeic acid; 50-81-7/Ascorbic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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