| Antioxidant reactivity toward nitroxide probes anchored into human serum albumin. A new model for studying antioxidant repairing capacity of protein radicals. | |
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MedLine Citation:
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PMID: 19804973 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new strategy to evaluate accessibility of antioxidants to radical proteins has been developed using nitroxide prefluorescent probes anchored into human serum albumin (HSA). Binding association constants for the nitroxide probes C(343)T and QT with HSA were 5 x 10(4) and 9 x 10(4)M(-1), respectively. Rate constants for the nitroxide reduction by antioxidants in HSA were determined finding k(HSA)/k(buffer) ratio of 0.8, 1.9, and 0.075 for ascorbic acid, Trolox, and caffeic acid, respectively, for the nitroxide C(343)T reduction. |
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Authors:
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Alexis Asp??e; Alejandra Orrego; Emilio Alarc??n; Camilo L??pez-Alarc??n; Horacio Poblete; Danilo Gonz??lez-Nilo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-22 |
Journal Detail:
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Title: Bioorganic & medicinal chemistry letters Volume: 19 ISSN: 1464-3405 ISO Abbreviation: Bioorg. Med. Chem. Lett. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-10-26 Completed Date: 2010-01-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9107377 Medline TA: Bioorg Med Chem Lett Country: England |
Other Details:
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Languages: eng Pagination: 6382-5 Citation Subset: IM |
Affiliation:
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Facultad de Qu??mica y Biolog??a, Universidad de Santiago de Chile, Casilla 40 Correo 33, Santiago, Chile. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Albumins
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physiology* Anaerobiosis / physiology Antioxidants / metabolism* Ascorbic Acid / metabolism* Caffeic Acids / pharmacology* Humans Nitric Oxide / metabolism Serum Albumin / drug effects, metabolism* Thermodynamics |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 0/Antioxidants; 0/Caffeic Acids; 0/Serum Albumin; 10102-43-9/Nitric Oxide; 331-39-5/caffeic acid; 50-81-7/Ascorbic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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