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Antioxidant profile and in vitro cardiac radical-scavenging vs pro-oxidant effects of commercial red grape juices (Vitis vinifera L. cv. Aglianico N.).
MedLine Citation:
PMID:  22917058     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Several works have reported on the pharmaceutical usefulness of grape phytochemicals. Nevertheless, scientific literature needs further studies to consider grape extracts as useful dietary supplements. The aim of the present work was to hypothesize for the first time the use of whole commercial red grape juices as food supplements potentially useful against both physiological and induced cardiac oxidative stress. First of all, our results indicated a good antioxidant stability of the juice sample to lyophilisation that may be reasonably regarded as a suitable process for the formulation of food supplements. Then, the processed sample (lioRGJ) was tested on cardiac derived H9C2 myocytes in order to ascertain its effects on reactive oxygen species (ROS) generation and caspase-3 activity incubating cardiomyocytes with lioRGJ at increasing doses (0.01-1 µg). Experiments showed an appreciable direct radical scavenging activity at a maximum sample dose of 0.01 µg that made the caspase-3 activity decrease by about 47% (P < 0.001). Cardiac cells were exposed to 1 μM doxorubicin and its combination with different doses of lioRGJ. Maximum sample aliquot of 0.01 µg seemed to effectively contrast the induced oxidant injury decreasing the ROS levels by about 31% and depressing the caspase-3 activity by about 60% (P < 0.001). In both assays, pro-oxidant effects at higher sample concentrations were detected as indicated by the increase in both ROS generation and apoptotic activity. Our data suggested for the juice sample the possibility to be employed as a food supplement with prospective cardioprotective benefits although further studies are needed to optimize its dosages in order to avoid harmful pro-oxidant effects.
Authors:
Gian Carlo Tenore; Michele Manfra; Paola Stiuso; Luigi Coppola; Mariateresa Russo; Isabel Maria Gomez Monterrey; Pietro Campiglia
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-23
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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