Document Detail


Antioxidant and phenolic acid profiles of tissue cultured and acclimatized Merwilla plumbea plantlets in relation to the applied cytokinins.
MedLine Citation:
PMID:  23747061     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Merwilla plumbea (Lindl.) Speta is an important medicinal plant widely used in traditional medicine. We evaluated the effect of five cytokinins [benzyladenine (BA), 2-isopentenyladenine (2iP), meta-topolin (mT), meta-topolin riboside (mTR), and meta-methoxy-9-tetrahydropyran-2-yl-topolin (MemTTHP)] on the level of phenolic acids and antioxidant activity of M. plumbea during the tissue culture and acclimatization stages. Two cytokinins (mT and mTR) significantly improved the antioxidant activity of tissue culture plantlets while the control plantlets were better after acclimatization. Using UPLC-MS/MS, the levels of hydroxybenzoic and hydroxycinnamic acid derivatives (phenolic acids) varied significantly during tissue culture and acclimatization, depending on the cytokinin and plant part analyzed. Vanillic acid (24.9μgg(-1)) detected in underground parts of tissue culture plants supplemented with BA was the most abundant phenolic acid detected. The current findings indicate that the phytochemicals together with the bioactivity during in vitro propagation of M. plumbea is influenced by the cytokinin type used and the stage of plant material collection.
Authors:
Adeyemi O Aremu; Jiří Gruz; Michaela Subrtová; Lucie Szüčová; Karel Doležal; Michael W Bairu; Jeffrey F Finnie; Johannes Van Staden
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-6-7
Journal Detail:
Title:  Journal of plant physiology     Volume:  -     ISSN:  1618-1328     ISO Abbreviation:  J. Plant Physiol.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-6-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882059     Medline TA:  J Plant Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Elsevier GmbH. All rights reserved.
Affiliation:
Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.
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