| Antioxidant enzyme inhibitors enhance singlet oxygen-induced cell death in HL-60 cells. | |
| | |
MedLine Citation:
|
PMID: 17018401 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Singlet oxygen is a highly reactive form of molecular oxygen that may harm living systems by oxidizing critical cellular macromolecules and it also promotes deleterious processes such as cell death. The protective role of antioxidant enzymes against singlet oxygen-induced oxidative damage in HL-60 cells was investigated in control and cells pre-treated with diethyldithiocarbamic acid, aminotriazole and oxlalomalate, specific inhibitors of superoxide dismutase, catalase and NADP+-dependent isocitrate dehydrogenase, respectively. Upon exposure to rose bengal (20 microM)/light (15 min), which generates singlet oxygen, to HL-60 cells, the viability was lower and the lipid peroxidation and oxidative DNA damage were higher in inhibitor-treated cells as compared to control cells. We also observed the significant increase in the endogenous production of reactive oxygen species as well as the significant decrease in the intracellular GSH level in inhibitor-treated HL-60 cells exposed to singlet oxygen. Upon exposure to rose bengal (3 microM)/light (15 min), which induced apoptotic cell death, a clear inverse relationship was observed between the control and inhibitor-treated HL-60 cells in their susceptibility to apoptosis. These results suggest that antioxidant enzymes play an important role in cellular defense against singlet oxygen-induced cell death including necrosis and apoptosis. |
| | |
Authors:
|
Sun Yee Kim; Su Min Lee; Jeen-Woo Park |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Free radical research Volume: 40 ISSN: 1071-5762 ISO Abbreviation: Free Radic. Res. Publication Date: 2006 Nov |
Date Detail:
|
Created Date: 2006-10-04 Completed Date: 2007-02-07 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: England |
Other Details:
|
Languages: eng Pagination: 1190-7 Citation Subset: IM |
Affiliation:
|
School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Taegu, 702-701, South Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antioxidants
/
metabolism,
pharmacology* Apoptosis Cell Death Cell Separation DNA Damage Enzyme Inhibitors / pharmacology* Glutathione / metabolism HL-60 Cells Humans Lipid Peroxidation Necrosis Oxidation-Reduction Oxidative Stress Oxygen / metabolism* Singlet Oxygen / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 0/Enzyme Inhibitors; 17778-80-2/Singlet Oxygen; 70-18-8/Glutathione; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Detection of risk-identifying chromosomal abnormalities and genomic profiling by multiplex ligation-...
Next Document: Gas-phase deposition of polychlorinated biphenyls (PCBs) to a water surface sampler.