Document Detail


Antioxidant effects of vitamins C and e on the low-density lipoprotein oxidation mediated by myeloperoxidase.
MedLine Citation:
PMID:  23279831     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Oxidative modification of low-density lipoprotein (LDL) appears to be an early step in the pathogenesis of atherosclerosis. Meanwhile, myeloperoxidase (MPO)-catalyzed reaction is one of the potent pathway for LDL oxidation in vivo. The aim of this study was to evaluate in vitro antioxidant effects of vitamins C and E on LDL oxidation mediated by MPO.
METHODS: MPO was isolated from fresh plasma by sequential centrifugation using density ultracentrifugation. It was incubated with LDL and the LDL oxidation level was determined spectrophotometrically by measuring conjugated diene absorbance at 234 nm. Furthermore, vitamin C (50-200 mM) and vitamin E (10-40 mM) were added and the LDL oxidation level was determined.
RESULTS: The purity index of MPO and its enzymatic activity were 0.69 and 1127 U/mg protein, respectively. It was demonstrated that vitamin C in vitro inhibited LDL oxidation mediated by MPO; however, vitamin E was unable to act in the same way. The protection by vitamin C was concentration dependent and maximum protective effect of vitamin C was observed at 150 mM, where about 64% of the LDL oxidation was inhibited. Vitamin C increased lag time of LDL oxidation mediated by MPO up to 2.4 times.
CONCLUSION: It can be concluded from our results that vitamin C is able to improve LDL resistance to oxidative modification in vitro. In addition, vitamin C might be effective in LDL oxidation mediated by MPO in vivo, resulting in reduction of atherosclerosis process rate.
Authors:
Seyed Ziyae Aldin Samsam Shariat; Sayed Abolfazl Mostafavi; Farzad Khakpour
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Iranian biomedical journal     Volume:  17     ISSN:  2008-823X     ISO Abbreviation:  Iran. Biomed. J.     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-02     Completed Date:  2013-06-25     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  9814853     Medline TA:  Iran Biomed J     Country:  Iran    
Other Details:
Languages:  eng     Pagination:  22-8     Citation Subset:  IM    
Affiliation:
School of Pharmacy and Pharmaceutical Sciences and Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan,
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / pharmacology
Ascorbic Acid / pharmacology*
Atherosclerosis / pathology
Humans
Lipid Peroxidation / drug effects
Lipoproteins, LDL / blood,  drug effects,  metabolism*
Oxidation-Reduction
Peroxidase / metabolism*
Vitamin E / pharmacology*
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Lipoproteins, LDL; 1406-18-4/Vitamin E; 50-81-7/Ascorbic Acid; EC 1.11.1.7/Peroxidase
Comments/Corrections

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