| Antioxidant effects of phlorotannins isolated from Ishige okamurae in free radical mediated oxidative systems. | |
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MedLine Citation:
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PMID: 18616277 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Three phlorotannins, including phloroglucinol, diphlorethohydroxycarmalol, and 6,6'-bieckol, were isolated from Ishige okamurae by column chromatography. The structures of the phlorotannins were determined on the basis of spectroscopic analysis, including NMR and mass spectrometry (MS) techniques. Antioxidant effects of phlorotannins were measured by direct free radical scavenging activities using the electron spin resonance spectrometry (ESR) technique and cellular systems in vitro. The results indicated that diphlorethohydroxycarmalol and 6,6'-bieckol showed potential radical scavenging activities against the 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, alkyl, and superoxide radicals. Moreover, no cytotoxicities of the phlorotannins on human fetal lung fibroblasts cell line (MRC-5), mouse macrophages cell line (RAW264.7), and human leukemic cell line (HL-60) were observed. In addition, diphlorethohydroxycarmalol and 6,6'-bieckol significantly reduced the intracellular reactive oxygen species level assessed by 2',7'-dichlorofluorescein diacetate (DCFH-DA) assay in RAW264.7 cells, and myeloperoxide (MPO) activity in HL-60 cells and radical-mediated oxidation of cell membrane proteins in RAW264.7 cells were dose-dependently inhibited in the presence of diphlorethohydroxycarmalol and 6,6'-bieckol. In conclusion, these results suggested that phlorotannins could be used as novel functional foodstuffs or antioxidants in the cosmetic and drug industries. |
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Authors:
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Yanping Zou; Zhong-Ji Qian; Yong Li; Moon-Moo Kim; Sang-Hoon Lee; Se-Kwon Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-11 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 56 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-20 Completed Date: 2008-10-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 7001-9 Citation Subset: IM |
Affiliation:
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Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, South Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algae, Brown
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chemistry* Animals Antioxidants / pharmacology* Cell Death / drug effects Cell Line Dioxins / isolation & purification, pharmacology* Electron Spin Resonance Spectroscopy Fibroblasts / drug effects HL-60 Cells Heterocyclic Compounds, 3-Ring / isolation & purification, pharmacology* Humans Macrophages / drug effects Magnetic Resonance Spectroscopy Mice Phloroglucinol / isolation & purification, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Dioxins; 0/Heterocyclic Compounds, 3-Ring; 0/diphlorethohydroxycarmalol; 108-73-6/Phloroglucinol; 88095-81-2/6,6'-bieckol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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