Document Detail

Antioxidant effect of phenelzine on MPP+-induced cell viability loss in differentiated PC12 cells.
MedLine Citation:
PMID:  14649725     Owner:  NLM     Status:  MEDLINE    
Phenelzine, deprenyl, and antioxidants (SOD, catalase, ascorbate, or rutin) reduced the loss of cell viability in differentiated PC12 cells treated with 250 microM MPP+, whereas N-acetylcysteine and dithiothreitol did not inhibit cell death. Phenelzine reduced the condensation and fragmentation of nuclei caused by MPP+ in PC12 cells. Phenelzine and deprenyl prevented the MPP+-induced decrease in mitochondrial membrane potential, cytochrome c release, formation of reactive oxygen species, and depletion of GSH in PC12 cells. Phenelzine revealed a scavenging action on hydrogen peroxide and reduced the hydrogen peroxide-induced cell death in PC12 cells, whereas deprenyl did not depress the cytotoxic effect of hydrogen peroxide. Both compounds reduced the iron and EDTA-mediated degradation of 2-deoxy-D-ribose degradation. The results suggest that phenelzine attenuates the MPP+-induced viability loss in PC12 cells by reducing the alteration of mitochondrial membrane permeability that seems to be mediated by oxidative stress.
Chung Soo Lee; Eun Sook Han; Won Bok Lee
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neurochemical research     Volume:  28     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-01     Completed Date:  2004-02-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1833-41     Citation Subset:  IM    
Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, South Korea.
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MeSH Terms
1-Methyl-4-phenylpyridinium / pharmacology*
Antioxidants / pharmacology*
Cell Differentiation / drug effects*
Cell Nucleus / drug effects
Cell Survival / drug effects*
Hydrogen Peroxide / pharmacology
PC12 Cells
Phenelzine / pharmacology*
Reactive Oxygen Species
Reg. No./Substance:
0/Antioxidants; 0/Reactive Oxygen Species; 48134-75-4/1-Methyl-4-phenylpyridinium; 51-71-8/Phenelzine; 7722-84-1/Hydrogen Peroxide

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