Document Detail

Antioxidant effect of conjugated linoleic acid and vitamin A during non enzymatic lipid peroxidation of rat liver microsomes and mitochondria.
MedLine Citation:
PMID:  12962148     Owner:  NLM     Status:  MEDLINE    
In the study reported here the effect of conjugated linoleic acid (CLA) and vitamin A on the polyunsaturated fatty acid composition, chemiluminescence and peroxidizability index of microsomes and mitochondria isolated from rat liver was analyzed. The effect of CLA on the polyunsaturated fatty acid composition of native microsomes was evidenced by an statistically significant p < 0.007 decrease of linoleic acid C18:2 n6, whereas in mitochondria it was observed a decrease p < 0.0001 of arachidonic acid C20:4 n6 when compared with vitamin A and control groups. Docosahexaenoic acid C22:6 n3 in mitochondria was reduced p < 0.04 in CLA and vitamin A groups when compared with control. After incubation of microsomes or mitochondria in an ascorbate (0.4 mM)-Fe++ (2.15 microM) system (120 min at 37 degrees C) it was observed that the total cpm/mg protein originated from light emission: chemiluminescence was lower in liver microsomes or mitochondria obtained from CLA group (received orally: 12.5 mg/daily during 10 days) than in the vitamin A group (received intraperitoneal injection: daily 0.195 g/ kg during 10 days). CLA reduced significantly maximal induced chemiluminescence in microsomes relative to vitamin A and control groups, whereas in mitochondria the effect was observed relative to control group. The polyunsaturated fatty acid composition of liver microsomes or mitochondria changed by CLA and vitamin A treatment. The polyunsaturated fatty acids mainly affected when microsomes native and peroxidized from control group were compared were linoleic, linolenic and arachidonic acids, while in vitamin A group linoleic and arachidonic acid were mainly peroxidized, whereas in CLA group only arachidonic acid was altered. In mitochondria obtained from the three groups arachidonic acid and docosahexaenoic acid showed a significant decrease when native and peroxidized groups were compared. As a consequence the peroxidizability index, a parameter based on the maximal rate of oxidation of fatty acids, show significant changes in the CLA group compare vitamin A and control groups. The simultaneous analysis of peroxidizability index, chemiluminescence and fatty acid composition demonstrated that CLA is more effective than vitamin A protecting microsomes or mitochondria from peroxidative damage.
Alejandro Palacios; Viviana Piergiacomi; Angel Catalá
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  250     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-09-09     Completed Date:  2004-05-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  107-13     Citation Subset:  IM    
Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina.
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MeSH Terms
Antioxidants / metabolism*
Arachidonic Acid / metabolism
Docosahexaenoic Acids / chemistry
Fatty Acids / metabolism
Fatty Acids, Unsaturated / metabolism
Linoleic Acids, Conjugated / chemistry*
Lipid Peroxidation
Liver / metabolism
Microsomes, Liver / metabolism*
Mitochondria / metabolism*
Rats, Wistar
Vitamin A / chemistry*,  metabolism
Reg. No./Substance:
0/Antioxidants; 0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Linoleic Acids, Conjugated; 11103-57-4/Vitamin A; 25167-62-8/Docosahexaenoic Acids; 506-32-1/Arachidonic Acid

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