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Antioxidant defense is one of the mechanisms by which mosquito cells survive dengue 2 viral infection.
MedLine Citation:
PMID:  21216424     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Dengue viruses (DENVs) generally induce apoptosis in mammalian cells but cause only minor damage in mosquito cells. To find genes involved in determining the cell fate, datasets derived from expressed sequence tags (ESTs) of C6/36 cells with and without infection were established. Of overexpressed genes found in infected dataset, chaperone proteins were validated significantly upregulated in C6/36 cells at 24hpi. It suggests that DENV-2 in mosquito cells activates the unfolded protein response to cope with endoplasmic reticular stress. Changes in the mitochondrial membrane potential and generation of superoxide provided further evidence that DENV-2 induces oxidative stress in both C6/36 and BHK-21 cells. Significant elevation of glutathione S-transferase (GST) activity was shown in infected C6/36, but not BHK-21, cells, while suppression of GST produced superoxide at 36hpi and increased the cell death rate at 48hpi. This indicates that mosquito cells protect themselves against viral infection through antioxidant defenses. RESEARCH HIGHLIGHTS: ► Elucidation on the response to DENV infection between mosquito and mammalian cells. ► Identification of ER-associated chaperones based on EST libraries of mosquito cells. ► Mosquito cells rescue themselves from DENV infection through antioxidant defenses. ► The first evidence of oxidative stress in mosquito cells against of DENV infection.
Authors:
Tien-Huang Chen; Petrus Tang; Chao-Fu Yang; Lin-Hsien Kao; Yin-Ping Lo; Ching-Kai Chuang; Yu-Tzu Shih; Wei-June Chen
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Publication Detail:
Type:  Journal Article     Date:  2011-01-08
Journal Detail:
Title:  Virology     Volume:  410     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  410-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Graduate Institute of Biomedical Sciences, Chang Gung University, Kwei-San, Tao-Yuan, 33332, Taiwan.
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