Document Detail


Antioxidant activity of peptide fractions derived from cottonseed protein hydrolysate.
MedLine Citation:
PMID:  20602516     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Cottonseed protein is widely regarded as a potential source of nutrients for humans and animals, but it is mainly used as forage in China. In the present study, Neutrase was employed to hydrolyse cottonseed protein to produce a hydrolysate with antioxidant activity suitable for conversion to high-value products. The antioxidant potential of the cottonseed protein hydrolysate (CPH) and its fractions was investigated using different in vitro methods. Furthermore, the amino acid composition of the CPH fractions was determined to evaluate the relationship between antioxidant activity and amino acid composition.
RESULTS: The CPH prepared using Neutrase was separated into four fractions (I, II, III and IV) by gel filtration on Sephadex G-25. All fractions were effective antioxidants, with fraction III (0.8-1.2 kDa) showing the strongest activity. The amino acid analysis showed that fraction III also had the highest total amino acid content (616.8 g kg(-1) protein) and was rich in Phe, His, Pro, Met, Ile and Cys compared with the other fractions.
CONCLUSION: The results showed that the hydrolysate derived from cottonseed protein, particularly fraction III, could be a natural antioxidant source suitable for use as a food additive.
Authors:
Dandan Gao; Yusheng Cao; Haixing Li
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of the science of food and agriculture     Volume:  90     ISSN:  1097-0010     ISO Abbreviation:  J. Sci. Food Agric.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-12     Completed Date:  2011-01-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376334     Medline TA:  J Sci Food Agric     Country:  England    
Other Details:
Languages:  eng     Pagination:  1855-60     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Society of Chemical Industry.
Affiliation:
State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*
Antioxidants / chemistry,  pharmacology*
Free Radicals / metabolism
Gossypium / chemistry*
Hydrolysis
Peptides / chemistry,  pharmacology*
Plant Proteins / chemistry*
Seeds
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Antioxidants; 0/Free Radicals; 0/Peptides; 0/Plant Proteins

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