| Antioxidant activity of mangostin in cell-free system and its effect on K562 leukemia cell line in photodynamic therapy. | |
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MedLine Citation:
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PMID: 20011978 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mangostin (MAG), a kind of xanthone widely used in diet and medicine, has antioxidant, anti-inflammatory, antimicrobial, and anticancer activities. On account of its antioxidant activity, MAG might protect cancer cells from free radical damage in photodynamic therapy (PDT) during which reactive oxygen species production was stimulated leading to irreversible tumor cell injury. In this study, the antioxidant activity of MAG was investigated and the influence of MAG on K562 cells in 5-aminolevulinic acid (ALA)-based PDT is demonstrated. The results showed that MAG could scavenge hydroxyl radical, superoxide anion, and hydrogen peroxide and inhibit the formation of malondialdehyde (MDA), but increase the amounts of singlet oxygen in cell-free systems. MAG inhibits cell proliferation and enhances cell apoptosis, lipid peroxidation, and DNA damage in ALA-PDT on K562 cells. NaN3, a singlet oxygen quencher, suppresses the MAG-induced cell apoptosis, lipid peroxidation, and DNA damage. In conclusion, MAG enhances the PDT-induced cytotoxicity in K562 cells and singlet oxygen was involved in this process. These results implied that the effect of antioxidants on PDT might be determined by its sensitization ability to singlet oxygen. |
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Authors:
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Dan Sun; Sujuan Zhang; Yongfeng Wei; Lingfang Yin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta biochimica et biophysica Sinica Volume: 41 ISSN: 1745-7270 ISO Abbreviation: Acta Biochim. Biophys. Sin. (Shanghai) Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-16 Completed Date: 2010-03-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101206716 Medline TA: Acta Biochim Biophys Sin (Shanghai) Country: China |
Other Details:
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Languages: eng Pagination: 1033-43 Citation Subset: IM |
Affiliation:
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Institute of Photonics and Phototechnology, Northwest University, Xi'an 710069, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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pharmacology* Apoptosis / drug effects Cell-Free System DNA Damage Humans K562 Cells Leukemia / drug therapy*, metabolism, pathology* Lipid Peroxidation / drug effects, physiology Photochemotherapy* Reactive Oxygen Species / metabolism Sodium Azide / pharmacology Xanthones / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Reactive Oxygen Species; 0/Xanthones; 26628-22-8/Sodium Azide; 6147-11-1/mangostin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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