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Antioxidant activity and identification of bioactive compounds from leaves of Anthocephalus cadamba by ultra-performance liquid chromatography/electrospray ionization quadrupole time of flight mass spectrometry.
MedLine Citation:
PMID:  23199718     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
OBJECTIVE: To evaluate the antioxidant potential of different extract/fractions of Anthocephalus cadamba (A. cadamba) (Roxb.) Miq. (Rubiaceae) and study the tentative identification of their active constituents.
METHODS: The extract/fractions were screened for antioxidant activity using various in vitro assays viz. DPPH assay, ABTS assay, superoxide anion radical scavenging assay, reducing power assay and plasmid DNA nicking assay. Total phenolic content of extract/fractions was determined by colorimetric method. An ultra-performance LC-electrospray-quadrupole-time of flight mass spectrometry method was used to analyse the active constituents of extract/fractions of A. cadamba.
RESULTS: The ethyl acetate fraction was found to be most active fraction in all the assays as compared to other extract/fractions. The IC(50) value of ethyl acetate fraction (ETAC fraction) was 21.24 μg/mL, 1.12 μg/mL, 9.68 μg/mL and 57.81 μg/mL in DPPH assay, ABTS assay, reducing power assay and superoxide scavenging assay respectively. All the extract/fractions also showed the potential to protect the plasmid DNA (pBR322) against the attack of hydroxyl radicals generated by Fentońs reagent. The bioactive compounds were identified by UPLC-ESI-QTOF-MS, by comparing the mass and λ(max) with literature values.
CONCLUSIONS: The potential of the extract/fractions to scavenge different free radicals in different systems indicated that they may be useful therapeutic agents for treating radical-related pathologic damage.
Authors:
Madhu Chandel; Upendra Sharma; Neeraj Kumar; Bikram Singh; Satwinderjeet Kaur
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Asian Pacific journal of tropical medicine     Volume:  5     ISSN:  1995-7645     ISO Abbreviation:  Asian Pac J Trop Med     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101533720     Medline TA:  Asian Pac J Trop Med     Country:  Singapore    
Other Details:
Languages:  eng     Pagination:  977-85     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Hainan Medical College. Published by Elsevier B.V. All rights reserved.
Affiliation:
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar-143005 India.
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