| The Antioxidant Cerebralcare Granule(R) Attenuates Cerebral Microcirculatory Disturbance During Ischemia-Reperfusion Injury. | |
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MedLine Citation:
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PMID: 19060781 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Cerebralcare Granule(R) (CG) is a compound Chinese medicine used for treatment of headache and dizziness associated with cerebrovascular diseases. To clarify the mechanism underlying the clinical outcome of CG, this study investigated the effects of CG on the structure and function of cerebral microvasculature during ischemia and reperfusion injury (I/R). A total of 138 Mongolian gerbils were included and divided into 4 groups, each 36 or 30 animals, for evaluating various parameters of concern. A skull window was prepared for microcirculatory observation in animals, which were subjected to ischemia-reperfusion with or without pretreatment with CG (0.4 g/kg or 0.8 g/kg). The velocity of RBCs in the venules was observed by a high speed video camera system, along with intravital confocal microscopic measurements of microvascular diameters, adherent leukocytes, and albumin leakage in the brain cortex. Changes in the fluorescence intensity of dihydrorhodamine 123 (DHR) in cerebral microvessels and malondialdehyde (MDA) level in cortex were measured. The ultrastructure of the microvessels in the cerebral cortex was analyzed using both transmission and scanning electron microscopy. In addition, cerebral blood flow was monitored using the laser Doppler imaging technique. Pretreatment with CG (0.4 g/kg or 0.8 g/kg) significantly alleviated I/R induced disorders in cerebral microvasculature, as evidenced by the data observed at 60 min of reperfusion wherein the values in CG (0.4 g/kg) pretreatment group, CG (0.8 g/kg) pretreatment group and I/R group were 2.43 +/- 0.24, 2.28 +/- 0.18 and 6.00 +/- 0.35 for leukocyte adhesion, 2.51 +/- 0.40, 2.33 +/- 0.29 and 4.77 +/- 0.24 for albumin leakage, 7.06 +/- 0.81, 5.93 +/- 0.42 and 28.38 +/- 2.70 for DHR fluorescence intensity in cerebral microvessels, 16.35 +/- 0.52, 14.34 +/- 0.68 and 21.46 +/- 0.71 for MDA level in cortex, and 0.43 +/- 0.07, 0.46 +/- 0.02 and 0.17 +/- 0.08 for cerebral blood flow, respectively. I/R elicited ultrastructural alterations in microvessels in cerebral cortex were also mitigated impressively by CG administration, manifested as attenuation of the reduced number of opening capillaries and the altered fine structures in endothelium, which were characterized by rough inner surface, increased intracellular vesicles, hypertrophy of digitations of intercellular contact, and swollen perivascular astroglial processes. CG is able to attenuate I/R-induced functional and structural changes in microvessels in cerebral cortex of gerbils, an ability that is most likely correlated with its antioxidant potential. |
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Authors:
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Xiang-Shun Xu; Zhi-Zhong Ma; Fang Wang; Bai-He Hu; Chuan-She Wang; Yu-Ying Liu; Xin-Rong Zhao; Li-Hua An; Xin Chang; Fu-Long Liao; Jing-Yu Fan; Hideyuki Niimi; Jing-Yan Han |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2008-12-4 |
Journal Detail:
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Title: Shock (Augusta, Ga.) Volume: - ISSN: 1540-0514 ISO Abbreviation: Shock Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-12-8 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9421564 Medline TA: Shock Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1)Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China, 2)Department of Integration of Chinese and Western Medicine, Peking University Health Science Center, Beijing, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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