| Antinociceptive effect of intrathecal cannabinoid receptor agonist WIN 55,212-2 in a rat bone tumor pain model. | |
| | |
MedLine Citation:
|
PMID: 21195743 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Bone tumor pain is a poorly controlled pain comprising background and severe pain on moving or weight-bearing postures that decreases the quality of life for cancer patients; thus, more effective analgesics are clearly needed. This study evaluated the efficacy of a cannabinoid (CB) receptor agonist (WIN 55,212-2) on bone tumor pain in the spinal cords of rats, and clarified the roles of the CB1 and CB2 receptors in WIN 55,212-2-induced antinociception at the spinal level. Bone tumor pain was induced by injecting MRMT-1 tumor cells (1×10(5)) into the right tibias of female Sprague-Dawley rats under sevoflurane anesthesia. Bone tumor development was monitored radiologically. Under sevoflurane anesthesia, a polyethylene catheter was inserted into the intrathecal space for drug administration. To assess pain, the withdrawal threshold was measured by applying a von Frey filament to the tumor cell inoculation site. The effect of intrathecal WIN 55,212-2 was investigated. Next, the WIN 55,212-2-mediated antinociception was reversed using CB1 (AM 251) and CB2 (AM 630) receptor antagonists. The intratibial injection of MRMT-1 tumor cells produced radiologically confirmed bone tumors. The paw withdrawal threshold decreased significantly (mechanical allodynia) with tumor development; however, intrathecal WIN 55,212-2 dose-dependently increased the withdrawal threshold. The antinociceptive effect of WIN 55,212-2 was reversed by both CB1 and CB2 receptor antagonists. Intrathecal WIN 55,212-2 reduced bone tumor-related pain behavior mediated via spinal CB1 and CB2 receptors. Therefore, spinal CB receptor agonists may be novel analgesics in the treatment of bone tumor pain. |
| | |
Authors:
|
Jin Hua Cui; Woong Mo Kim; Hyung Gon Lee; Ye Ok Kim; Chang Mo Kim; Myung Ha Yoon |
Related Documents
:
|
3532333 - Preclinical biology of alpha interferons. 19224633 - Synergistic up-regulation of mcp-2/ccl8 activity is counteracted by chemokine cleavage,... 7479353 - Endocrine tumors of the small and large intestine. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2010-12-31 |
Journal Detail:
|
Title: Neuroscience letters Volume: - ISSN: 1872-7972 ISO Abbreviation: - Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2011-1-11 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier Ireland Ltd. |
Affiliation:
|
The Brain Korea 21 Project, Center for Biomedical Human Resources at Chonnam National University, Gwangju, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Disruption of MT impairs motion processing.
Next Document: The general anesthetic sevoflurane affects the expression of clock gene mPer2 accompanying the chang...