Document Detail

Antinociceptive effect of different types of calcium channel inhibitors and the distribution of various calcium channel alpha 1 subunits in the dorsal horn of spinal cord in mice.
MedLine Citation:
PMID:  15451373     Owner:  NLM     Status:  MEDLINE    
To understand better which voltage-dependent calcium channels (VGCCs) are involved in nociceptive neurotransmission, we investigated the pharmacological properties and distribution of VGCCs in the mouse spinal cord. A behavioral assay revealed that intrathecal injections of omega-agatoxin TK, omega-agatoxin IVA, omega-conotoxin GVIA, and SNX-482, which block P/Q-, P/Q-, N-, and R-type calcium channels, respectively, produced analgesic effects, while an L-type channel blocker had no such effect. An electrophysiological study demonstrated the presence of various types of VGCCs within dorsal root ganglion (DRG) neurons. Immunohistochemistry revealed distinct localization of P/Q-, N-, L-, and R-type calcium channel subunits to the dorsal horn of the spinal cord. The results of this study revealed the localization and functions of several calcium channels that are involved in nociceptive neurotransmission within the dorsal horn of the mouse spinal cord.
Manabu Murakami; Osamu Nakagawasai; Takashi Suzuki; Izadi I Mobarakeh; Yumiko Sakurada; Astunobu Murata; Fumihiro Yamadera; Ichiro Miyoshi; Kazuhiko Yanai; Koichi Tan-No; Hironobu Sasano; Takeshi Tadano; Toshihiko Iijima
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research     Volume:  1024     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-09-28     Completed Date:  2005-01-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  122-9     Citation Subset:  IM    
Department of Pharmacology, Akita University School of Medicine, 1-1-1 Hondoh, Akita 010-8543, Japan.
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MeSH Terms
Analgesics / pharmacology*
Calcium Channel Blockers / pharmacology*
Calcium Channels / analysis,  physiology*
Pain Measurement / drug effects,  statistics & numerical data
Posterior Horn Cells / chemistry,  drug effects*,  physiology
Protein Subunits / analysis,  antagonists & inhibitors*,  physiology
Reg. No./Substance:
0/Analgesics; 0/Calcium Channel Blockers; 0/Calcium Channels; 0/Protein Subunits

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