Document Detail

Antinociceptive and anti-inflammatory effects of the saponin and sapogenins obtained from the stem of Akebia quinata.
MedLine Citation:
PMID:  15857214     Owner:  NLM     Status:  MEDLINE    
The stem of Akebia quinata Decasisne (Lardizabalaceae) has been used to treat urinary tract inflammatory disease. It has been reported that saponins in medicinal plants may act as bioactive components after biodegradation to sapogenins in the gastrointestinal tract. To find the active components, we obtained the methanol (MeOH) extract from A. quinata stems and fractionated this extract into CHCl(3), butanol (BuOH), and H(2)O fractions. A saponin-containing BuOH fraction was refluxed in an acidic solution to yield the hydrolyzed fraction. Silica gel column chromatography separated kalopanaxsaponin A (1) from the BuOH fraction, and oleanolic acid (2) and hederagenin (3) were obtained from the hydrolyzed fraction. The antinociceptive effect was tested by hot plate-writhing and tail-flicks methods using mice, and the anti-inflammatory effect was assayed using carrageenan-induced rat edema against the following samples: the MeOH extract of A. quinata stems, its fractions, the isolated saponin, kalopanaxsaponin A, and the sapogenins hederagenin and oleanolic acid. The MeOH extract exhibited antinociceptive/anti-inflammatory effects by oral administration of 100 and 250 mg/kg doses, indicating that the MeOH extract has an antinociceptive/anti-inflammatory activity. The BuOH fraction (crude saponin) also significantly exhibited those bioactivities. Treatments with 10 and 30 mg/kg perorally of these two sapogenins produced significant antinociceptive/ anti-inflammatory effects in the rat, suggesting that the sapogenins may act as resultant active compounds. Compounds 2 and 3 inhibited dye leakage into the peritoneal cavity induced by acetic acid, and the latter was more active than the former. The anti-inflammtory effects were further supported by the reduction of carrageenan-induced lipid peroxidation and hydroxy radical content in serum. These results suggest that the antinociceptive/anti-inflammatory properties of the stem of A. quinata can be attributed to the sapogenins oleanolic acid and hederagenin.
Jongwon Choi; Hyun-Ju Jung; Kyung-Tae Lee; Hee-Juhn Park
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medicinal food     Volume:  8     ISSN:  1096-620X     ISO Abbreviation:  J Med Food     Publication Date:  2005  
Date Detail:
Created Date:  2005-04-28     Completed Date:  2005-08-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9812512     Medline TA:  J Med Food     Country:  United States    
Other Details:
Languages:  eng     Pagination:  78-85     Citation Subset:  IM    
College of Pharmacy, Kyungsung University, Pusan, Korea.
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MeSH Terms
Administration, Oral
Analgesics / pharmacology*
Angiosperms / chemistry*
Anti-Inflammatory Agents / pharmacology*
Butanols / chemistry
Dose-Response Relationship, Drug
Methanol / chemistry
Mice, Inbred ICR
Oleanolic Acid / analogs & derivatives*,  pharmacology*
Pain Measurement / drug effects
Plant Extracts / pharmacology*
Plant Stems / chemistry
Rats, Sprague-Dawley
Sapogenins / pharmacology
Saponins / pharmacology
Reg. No./Substance:
0/Analgesics; 0/Anti-Inflammatory Agents; 0/Butanols; 0/Plant Extracts; 0/Sapogenins; 0/Saponins; 0/kalopanax saponin A; 465-99-6/hederagenin; 508-02-1/Oleanolic Acid; 67-56-1/Methanol

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