Document Detail


Antimutagenic properties of probiotic bacteria and of organic acids.
MedLine Citation:
PMID:  9541641     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antimutagenic activities of live and killed cells of 6 strains of Lactobacillus acidophilus and 9 strains of bifidobacteria and of organic acids usually produced by these probiotic bacteria were determined using 8 potent chemical mutagens and promutagens. The mutagens and promutagens used were N-methyl, N'-nitro, N-nitrosoguanidine; 2-nitroflourene; 4-nitro-O-phenylenediamine; 4-nitroquinoline-N-oxide; Aflatoxin-B; 2-amino-3-methyl-3H-imidazoquinoline; 2-amino-1-methyl-6-phenyl-imidazo (4,5-b) pyridine, and 2-amino-3-methyl-9H-pyrido (3,3-6) indole. The mutagenicity of these mutagens and antimutagenic activity of probiotic bacteria against the mutagens were determined according to the Ames TA-100 assay using a mutant of Salmonella typhimurium. Efficiency of bacterial cells in binding or inhibiting these mutagens was also investigated. Live cells of probiotic bacteria showed higher antimutagenic activity and their efficiency in inhibiting the mutagens was better than killed bacterial cells. Live bacterial cells bound or inhibited the mutagens permanently, whereas killed bacteria released mutagens upon extraction with dimethyl sulfoxide. Among the organic acids, butyric acid showed highest inhibition of mutagens followed by acetic acid. Lactic and pyruvic acids did not show appreciable levels of inhibition.
Authors:
W E Lankaputhra; N P Shah
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mutation research     Volume:  397     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-04-22     Completed Date:  1998-04-22     Revised Date:  2006-04-13    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  169-82     Citation Subset:  IM    
Affiliation:
Centre for Bioprocessing and Food Technology, Victoria University of Technology, Melbourne, Australia.
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MeSH Terms
Descriptor/Qualifier:
Bifidobacterium / metabolism,  physiology*
Carboxylic Acids / metabolism*
Lactobacillus acidophilus / metabolism,  physiology*
Mutagens / toxicity*
Probiotics*
Salmonella typhimurium / genetics
Chemical
Reg. No./Substance:
0/Carboxylic Acids; 0/Mutagens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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