Document Detail


Antimutagenic activity of chemical fractions isolated from a commercial soybean processing by-product.
MedLine Citation:
PMID:  10332809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Commercial products of agronomic crop plants may become a reliable and inexpensive source of phytonutrients, such as antimutagenic food supplements. We previously demonstrated that PCC, an ethanol extract of a commercial soybean processing by-product, was able to repress induced genomic DNA damage, whole cell clastogenicity, and point mutation in mammalian cells. In this paper we separated PCC into a series of chemically defined fractions and determined their ability to repress induced mutagenic damage in Chinese hamster lung cells, Chinese hamster ovary cells and human lymphocytes. Fraction PCC70 (PCC 70% methanol eluate) contained the flavonoids from PCC and daidzin and genistin repressed 2-acetoxyacetylaminofluorene (2AAAF)-induced DNA damage measured with single cell gel electrophoresis. Genistein, however, enhanced the genotoxic impact of 2AAAF. Fraction PCC100 (PCC 100% methanol eluate) had the greatest level of antigenotoxic activity against 2AAAF in CHO cells and repressed the genotoxic capacity of the dietary carcinogen 2-amino-3-methylimidazo-(4,5-f)quinoline (IQ) in human lymphocytes. These data indicate that commercial soybean products and by-products may be a source of chemoprotective food additives.
Authors:
M J Plewa; E D Wagner; M A Berhow; A Conway; A L Rayburn; D Anderson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Teratogenesis, carcinogenesis, and mutagenesis     Volume:  19     ISSN:  0270-3211     ISO Abbreviation:  Teratog., Carcinog. Mutagen.     Publication Date:  1999  
Date Detail:
Created Date:  1999-08-11     Completed Date:  1999-08-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100917     Medline TA:  Teratog Carcinog Mutagen     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  121-35     Citation Subset:  IM    
Affiliation:
Department of Crop Sciences, College of Agricultural, Consumer and Environmental Sciences, University of Illinois at Urbana-Champaign, USA. m-plewa@uiuc.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Antimutagenic Agents / pharmacology*
CHO Cells
Cell Fractionation
Cricetinae
Dose-Response Relationship, Drug
Flow Cytometry
Food Handling*
Humans
Lymphocytes / drug effects
Mutagenicity Tests
Soybeans / metabolism*
Time Factors
Chemical
Reg. No./Substance:
0/Antimutagenic Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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