Document Detail


Antimicrobial resistance among invasive Haemophilus influenzae strains: results of a Brazilian study carried out from 1996 through 2000.
MedLine Citation:
PMID:  12426628     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A total of 1712 strains of Haemophilus influenzae isolated from patients with invasive diseases were obtained from ten Brazilian states from 1996 to 2000. beta-Lactamase production was assessed and the minimum inhibitory concentrations (MIC) of ampicillin, chloramphenicol, ceftriaxone and rifampin were determined using a method for broth microdilution of Haemophilus test medium. The prevalence of strains producing beta-lactamase ranged from 6.6 to 57.7%, with an overall prevalence of 18.4%. High frequency of beta-lactamase-mediated ampicillin resistance was observed in Distrito Federal (25%), São Paulo (21.7%) and Paraná (18.5%). Of the 1712 strains analyzed, none was beta-lactamase negative, ampicillin resistant. A total of 16.8% of the strains were resistant to chloramphenicol, and 13.8% of these also presented resistance to ampicillin, and only 3.0% were resistant to chloramphenicol alone. All strains were susceptible to ceftriaxone and rifampin and the MIC90 were 0.015 micro g/ml and 0.25 micro g/ml, respectively. Ceftriaxone is the drug of choice for empirical treatment of bacterial meningitis in pediatric patients who have not been screened for drug susceptibility. The emergence of drug resistance is a serious challenge for the management of invasive H. influenzae disease, which emphasizes the fundamental role of laboratory-based surveillance for antimicrobial resistance.
Authors:
S T Casagrande; I M Landgraf; A M M Kobata; R C Zanella; S Bokermann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]     Volume:  35     ISSN:  0100-879X     ISO Abbreviation:  Braz. J. Med. Biol. Res.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-11-11     Completed Date:  2003-04-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8112917     Medline TA:  Braz J Med Biol Res     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  1293-300     Citation Subset:  IM    
Affiliation:
Departamento de Bacteriologia, Instituto Adolfo Lutz, São Paulo, SP, Brasil.
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MeSH Terms
Descriptor/Qualifier:
Ampicillin Resistance
Anti-Bacterial Agents / pharmacology*
Brazil
Ceftriaxone / pharmacology
Child
Chloramphenicol Resistance
Drug Resistance, Bacterial
Haemophilus influenzae / drug effects*,  enzymology,  isolation & purification
Humans
Meningitis, Haemophilus / microbiology*
Microbial Sensitivity Tests
Rifampin / pharmacology
beta-Lactamases / biosynthesis*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 13292-46-1/Rifampin; 73384-59-5/Ceftriaxone; EC 3.5.2.6/beta-Lactamases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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