Document Detail


Antimicrobial defensin peptides of the human nasal mucosa.
MedLine Citation:
PMID:  11860065     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Defensins, a prominent group of antimicrobial peptides, are an important component of the innate immune response, particularly at mucosal surfaces that are vulnerable to colonization by potential pathogens. The present study was undertaken to investigate the expression of defensins in inferior turbinate mucosa of normal subjects and inferior turbinate mucosa and nasal polyps of patients with chronic sinusitis. Expression of beta-defensin 1 and 2 and alpha-defensin 5 and 6 messenger RNAs (mRNAs) was investigated by reverse transcriptase-polymerase chain reaction, and their expression level was semiquantitatively evaluated by dot blot hybridization. Immunohistochemical analysis was used for detection of alpha-defensins 1, 2, and 3 in tissue sections. Beta-defensin 1 mRNA was expressed in all tissue samples, at levels that did not differ significantly. Beta-defensin 2 mRNA was detected in the turbinate mucosa and nasal polyps of patients with chronic sinusitis, but not in normal mucosa. Its expression level was significantly higher in nasal polyps than in turbinate mucosa. Alpha-defensin 5 and 6 mRNAs were not expressed in any tissues, but alpha-defensins 1, 2, and 3 were detected in all tissue samples obtained from patients with chronic sinusitis. These results suggest that beta-defensin 1 may play a constitutive role in nasal defenses, whereas alpha-defensins 1, 2, and 3 and beta-defensin 2 may be induced in response to local infection or inflammation.
Authors:
Sang Hag Lee; Jung Eun Kim; Hyun Ho Lim; Heung Man Lee; Jong Ouck Choi
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Annals of otology, rhinology, and laryngology     Volume:  111     ISSN:  0003-4894     ISO Abbreviation:  Ann. Otol. Rhinol. Laryngol.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-02-25     Completed Date:  2002-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0407300     Medline TA:  Ann Otol Rhinol Laryngol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  135-41     Citation Subset:  AIM; IM    
Affiliation:
Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Korea University, Seoul.
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MeSH Terms
Descriptor/Qualifier:
Adult
Chronic Disease
Defensins / genetics,  metabolism*
Female
Humans
Immunoblotting
Immunohistochemistry
Male
Nasal Mucosa / immunology*,  metabolism
Nasal Polyps / immunology,  metabolism
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Sinusitis / immunology,  metabolism
Turbinates
Chemical
Reg. No./Substance:
0/Defensins; 0/RNA, Messenger

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