Document Detail

Antimicrobial activity of saponin-rich fraction from Camellia oleifera cake and its effect on cell viability of mouse macrophage RAW 264.7.
MedLine Citation:
PMID:  22430639     Owner:  NLM     Status:  Publisher    
BACKGROUND: As a by-product of oil production, several million tons of Camellia oleifera cake is discarded every year in China. The aim of this study was to evaluate the antimicrobial activity of a saponin-rich fraction isolated from C. oleifera cake and investigate its effect on the cell viability of RAW 264.7, a macrophage-like cell line present in almost all mouse tissues. RESULTS: The saponin-rich fraction was isolated from C. oleifera cake in several steps and had a saponin content of 95.42 ± 0.10% (w/w). It showed significant inhibitory activity against the bacteria Staphylococcus aureus, Escherichia coli and Bacillus subtilis and the fungi Mucor racemosus, Aspergillus oryzae, Rhizopus stolonifer, Saccharomyces cerevisiae and Penicillium glaucum, with minimum inhibitory concentrations of 31.3, 31.3, 62.5, 250, 250, 250, 31.3 and 125 µg ml(-1) respectively. In addition, mouse macrophage RAW 264.7 pretreated with the saponin-rich fraction (80-200 µg mL(-1) ) exhibited a significant loss of cell viability in a dose-dependent manner. CONCLUSION: These results may be useful for the future application of saponins from C. oleifera cake. However, the potential use of the saponin-rich fraction as an antimicrobial agent should be decided according to the target micro-organisms in order to avoid causing harm in humans. Copyright © 2012 Society of Chemical Industry.
Jie-Lun Hu; Shao-Ping Nie; Dan-Fei Huang; Chang Li; Ming-Yong Xie; Yin Wan
Related Documents :
19532859 - Efficient dyad-based organic solar cells with a highly crystalline donor group.
151479 - Contribution to the knowledge of the fine structure of chondrosarcoma of bone. with a n...
13481019 - The fine structural organisation of rous tumour cells.
22595769 - Overexpression of bone morphogenetic protein 4 in sto fibroblast feeder cells represses...
22624979 - Chlamydia co-opts the rod-shape determining proteins mreb and pbp2 for cell division.
21389049 - Cell type-specific filamin complex regulation by a novel class of hect ubiquitin ligase...
22502879 - Experimental research of rb94 gene transfection into retinoblastoma cells using ultraso...
9163319 - Rat basophilic leukaemia (rbl) cells overexpressing rab3a have a reversible block in an...
10803519 - Cdp/cut dna binding activity is down-modulated in granulocytes, macrophages and erythro...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-19
Journal Detail:
Title:  Journal of the science of food and agriculture     Volume:  -     ISSN:  1097-0010     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376334     Medline TA:  J Sci Food Agric     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Society of Chemical Industry.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  CD138-negative clonogenic cells are plasma cells but not B cells in some multiple myeloma patients.
Next Document:  Streaming Weekly Soap Opera Video Episodes to Smartphones in a Randomized Controlled Trial to Reduce...