Document Detail


Antimicrobial activity of cuticle and outer eggshell protein extracts from three species of domestic birds.
MedLine Citation:
PMID:  18409087     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The eggshell cuticle is the proteinaceous outermost layer of the eggshell which regulates water exchange and protects against entry of micro-organisms. In this study, we investigated the hypothesis that the cuticle may also reduce microbial contamination by providing a chemical defence. 2. Outer eggshell and cuticle protein was extracted from domestic chicken (Gallus gallus), duck (Anas platyrhynchos) and goose (Anser anser) eggs by HCl and urea treatment, respectively. Antimicrobial activity of the extracts against Gram-positive and Gram-negative bacteria was evaluated. 3. C-type lysozyme, ovotransferrin and ovocalyxin-32 were identified in all extracts by Western blotting. All extracts from all species demonstrated lysozyme enzymatic activity. Immobilised c-type lysozyme retained some enzymatic activity. Protein extracts demonstrated activity against Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis suggesting the action of antimicrobial proteins in addition to lysozyme. 4. The results suggest that the antimicrobial outer eggshell and cuticle proteins present in a number of avian species may be a mechanism which enhances avian reproductive success.
Authors:
O Wellman-Labadie; J Picman; M T Hincke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British poultry science     Volume:  49     ISSN:  1466-1799     ISO Abbreviation:  Br. Poult. Sci.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-04-14     Completed Date:  2008-05-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  15740290R     Medline TA:  Br Poult Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  133-43     Citation Subset:  IM    
Affiliation:
Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Infective Agents / pharmacology*
Bacillus subtilis / drug effects
Blotting, Western
Chickens*
Conalbumin / analysis,  pharmacology
Ducks*
Egg Proteins / analysis,  pharmacology*
Egg Shell / anatomy & histology,  chemistry
Electrophoresis, Polyacrylamide Gel
Enzymes, Immobilized
Geese*
Muramidase / analysis,  metabolism,  pharmacology
Pseudomonas aeruginosa / drug effects
Staphylococcus aureus / drug effects
Chemical
Reg. No./Substance:
0/Anti-Infective Agents; 0/Egg Proteins; 0/Enzymes, Immobilized; 0/chorion proteins; 0/ovocalyxin 32; 1391-06-6/Conalbumin; EC 3.2.1.17/Muramidase

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