| Antimicrobial activity of acidic xylo-oligosaccharides produced by family 10 and 11 endoxylanases. | |
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MedLine Citation:
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PMID: 12568925 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Acidic oligosaccharides were obtained from birchwood xylan by treatment with a Thermoascus aurantiacus family 10 and a Sporotrichum thermophile family 11 endoxylanases. The main difference between the products liberated by xylanases of family 10 and 11 concerned the length of the products containing 4-O-methyl-D-glucuronic acid. The xylanase from T. aurantiacus liberate from glucuronoxylan an aldotetrauronic acid as the shortest acidic fragment in contrast with the enzyme from S. thermophile, which liberated an aldopentauronic acid. Acidic xylooligosaccharides were separated from the hydrolysate by anion-exchange and size-exclusion chromatography (SEC) and the primary structure was determined by 13C NMR spectroscopy. The acidic xylo-oligosaccharides were tested against three Gram-positive and three Gram-negative aerobically grown bacteria, as well as against Helicobacter pylori. Aldopentauronic acid was proved more active against the Gram-positive bacteria and against H. pylori. |
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Authors:
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P Christakopoulos; P Katapodis; E Kalogeris; D Kekos; B J Macris; H Stamatis; H Skaltsa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of biological macromolecules Volume: 31 ISSN: 0141-8130 ISO Abbreviation: Int. J. Biol. Macromol. Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2003-02-05 Completed Date: 2003-09-08 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7909578 Medline TA: Int J Biol Macromol Country: England |
Other Details:
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Languages: eng Pagination: 171-5 Citation Subset: IM |
Affiliation:
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Biotechnology Laboratory, Department of Chemical Engineering, National Technical University of Athens, Zografou Campus, Athens 15780, Greece. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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pharmacology* Chromatography Chromatography, Thin Layer Dose-Response Relationship, Drug Endo-1,4-beta Xylanases Hydrolysis Magnetic Resonance Spectroscopy Oligosaccharides / pharmacology* Uronic Acids / chemistry Xylan Endo-1,3-beta-Xylosidase Xylans / pharmacology Xylosidases / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Oligosaccharides; 0/Uronic Acids; 0/Xylans; EC 3.2.1.-/Xylosidases; EC 3.2.1.32/Xylan Endo-1,3-beta-Xylosidase; EC 3.2.1.8/Endo-1,4-beta Xylanases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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