Document Detail

Antimicrobial activity of acidic xylo-oligosaccharides produced by family 10 and 11 endoxylanases.
MedLine Citation:
PMID:  12568925     Owner:  NLM     Status:  MEDLINE    
Acidic oligosaccharides were obtained from birchwood xylan by treatment with a Thermoascus aurantiacus family 10 and a Sporotrichum thermophile family 11 endoxylanases. The main difference between the products liberated by xylanases of family 10 and 11 concerned the length of the products containing 4-O-methyl-D-glucuronic acid. The xylanase from T. aurantiacus liberate from glucuronoxylan an aldotetrauronic acid as the shortest acidic fragment in contrast with the enzyme from S. thermophile, which liberated an aldopentauronic acid. Acidic xylooligosaccharides were separated from the hydrolysate by anion-exchange and size-exclusion chromatography (SEC) and the primary structure was determined by 13C NMR spectroscopy. The acidic xylo-oligosaccharides were tested against three Gram-positive and three Gram-negative aerobically grown bacteria, as well as against Helicobacter pylori. Aldopentauronic acid was proved more active against the Gram-positive bacteria and against H. pylori.
P Christakopoulos; P Katapodis; E Kalogeris; D Kekos; B J Macris; H Stamatis; H Skaltsa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of biological macromolecules     Volume:  31     ISSN:  0141-8130     ISO Abbreviation:  Int. J. Biol. Macromol.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2003-02-05     Completed Date:  2003-09-08     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7909578     Medline TA:  Int J Biol Macromol     Country:  England    
Other Details:
Languages:  eng     Pagination:  171-5     Citation Subset:  IM    
Biotechnology Laboratory, Department of Chemical Engineering, National Technical University of Athens, Zografou Campus, Athens 15780, Greece.
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MeSH Terms
Anti-Bacterial Agents / pharmacology*
Chromatography, Thin Layer
Dose-Response Relationship, Drug
Endo-1,4-beta Xylanases
Magnetic Resonance Spectroscopy
Oligosaccharides / pharmacology*
Uronic Acids / chemistry
Xylan Endo-1,3-beta-Xylosidase
Xylans / pharmacology
Xylosidases / chemistry*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Oligosaccharides; 0/Uronic Acids; 0/Xylans; EC 3.2.1.-/Xylosidases; EC Endo-1,3-beta-Xylosidase; EC,4-beta Xylanases

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