Document Detail

Antimicrobial activity of phenolics and glucosinolate hydrolysis products and their synergy with streptomycin against pathogenic bacteria.
MedLine Citation:
PMID:  20632977     Owner:  NLM     Status:  MEDLINE    
The purpose of the present study was to evaluate the in vitro antibacterial effects of different classes of important and common dietary phytochemicals (5 simple phenolics - tyrosol, gallic acid, caffeic acid, ferulic acid, and chlorogenic acid; chalcone - phloridzin; flavan-3-ol - (-) epicatechin; seco-iridoid - oleuropein glucoside; 3 glucosinolate hydrolysis products - allylisothiocyanate, benzylisothiocyanate and 2-phenylethylisothiocyanate) against Escherichia coli, Pseudomonas aeruginosa, Listeria monocytogenes and Staphylococcus aureus. Another objective of this study was to evaluate the effects of dual combinations of streptomycin with the different phytochemicals on antibacterial activity. A disc diffusion assay was used to evaluate the antibacterial activity of the phytochemicals and 3 standard antibiotics (ciprofloxacin, gentamicin and streptomycin) against the four bacteria. The antimicrobial activity of single compounds and dual combinations (streptomycin-phytochemicals) were quantitatively assessed by measuring the inhibitory halos. The results showed that all of the isothiocyanates had significant antimicrobial activities, while the phenolics were much less efficient. No antimicrobial activity was observed with phloridzin. In general P. aeruginosa was the most sensitive microorganism and L. monocytogenes the most resistant. The application of dual combinations demonstrated synergy between streptomycin and gallic acid, ferulic acid, chlorogenic acid, allylisothiocyanate and 2-phenylethylisothiocyanate against the Gram-negative bacteria. In conclusion, phytochemical products and more specifically the isothiocyanates were effective inhibitors of the in vitro growth of the Gram-negative and Gram-positive pathogenic bacteria. Moreover, they can act synergistically with less efficient antibiotics to control bacterial growth.
Maria J Saavedra; Anabela Borges; Carla Dias; Alfredo Aires; Richard N Bennett; Eduardo S Rosa; Manuel Simões
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medicinal chemistry (Shāriqah (United Arab Emirates))     Volume:  6     ISSN:  1875-6638     ISO Abbreviation:  Med Chem     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-10-14     Completed Date:  2011-05-19     Revised Date:  2012-03-14    
Medline Journal Info:
Nlm Unique ID:  101240303     Medline TA:  Med Chem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  174-83     Citation Subset:  IM    
LEPAE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal.
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MeSH Terms
Anti-Bacterial Agents / chemistry,  pharmacology*
Catechin / pharmacology
Cinnamates / pharmacology*
Drug Combinations
Drug Evaluation, Preclinical
Drug Synergism
Escherichia coli / drug effects
Gallic Acid / pharmacology*
Isothiocyanates / pharmacology*
Listeria monocytogenes / drug effects
Phenylethyl Alcohol / analogs & derivatives,  pharmacology
Phlorhizin / pharmacology
Pseudomonas aeruginosa / drug effects
Pyrans / pharmacology
Staphylococcus aureus / drug effects
Streptomycin / pharmacology*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Cinnamates; 0/Drug Combinations; 0/Isothiocyanates; 0/Pyrans; 149-91-7/Gallic Acid; 154-23-4/Catechin; 32619-42-4/oleuropein; 501-94-0/4-hydroxyphenylethanol; 57-92-1/Streptomycin; 60-12-8/Phenylethyl Alcohol; 60-81-1/Phlorhizin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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